Department of Restorative Dentistry, University at Buffalo, School of Dental Medicine, 235 Squire Hall, Buffalo, NY 14214, United States.
J Dent. 2010 Aug;38(8):621-5. doi: 10.1016/j.jdent.2009.08.011. Epub 2009 Sep 23.
The study evaluated the antibacterial effect of VIOlight (VL) Personal Travel Toothbrush Sanitizer on biofilms after toothbrush exposure to human saliva compared to Listerine Antiseptic (LA), 3% hydrogen peroxide (3%HP) and water.
Twenty toothbrush heads (n=5/Gp) were immersed in saliva and to allow for bacterial growth and biofilm formation for 24h. VL sanitizer and antiseptic(s) were used for 7 min; after treatment, brush heads were rinsed and placed into 10 mL of 2x AOAC Letheen Broth, sonicated and vortexed for 10s. Tenfold serial dilutions were prepared and plated and incubated aerobically and anaerobically. Log(10)CFU/mL data were compared utilizing ANOVA (p<0.05).
Results showed 3%HP with significantly lower counts than LA, VL and control for aerobic and anaerobic bacteria. LA had significantly lower counts than VL and control for both types of bacteria and VIOlight had significantly lower counts than the control for aerobic bacteria. 3%HP and LA were most effective in rapidly killing bacteria when compared to VIOlight.
Results showed that 3% hydrogen peroxide was most effective in reducing the numbers of both aerobic and anaerobic bacteria present on the toothbrush heads. Under the same test conditions, Listerine Antiseptic was shown to be secondarily effective for the same bacteria while the VIOlight unit was the least effective when compared to the other treatment groups.
本研究评估了 VIOlight（VL）个人旅行牙刷消毒器在牙刷暴露于唾液后与李施德林抗菌剂（LA）、3%过氧化氢（3%HP）和水相比对生物膜的抗菌效果。
将 20 个牙刷头（n=5/组）浸入唾液中，以允许细菌生长和生物膜形成 24 小时。VL 消毒剂和抗菌剂（s）使用 7 分钟；处理后，冲洗牙刷头并放入 10 毫升 2x AOAC Letheen 肉汤中，进行超声处理和涡旋 10 秒。制备十倍系列稀释液并进行平板培养，在需氧和厌氧条件下孵育。使用方差分析（p<0.05）比较 log(10)CFU/mL 数据。
结果表明，3%HP 的有氧和厌氧菌计数明显低于 LA、VL 和对照组。LA 的有氧和厌氧菌计数明显低于 VL 和对照组，而 VL 的有氧细菌计数明显低于对照组。与 VIOlight 相比，3%HP 和 LA 在快速杀死细菌方面更有效。
结果表明，3%过氧化氢在减少牙刷头上存在的有氧和厌氧菌数量方面最有效。在相同的测试条件下，李施德林抗菌剂对相同的细菌显示出其次效性，而 VIOlight 装置与其他治疗组相比效果最差。
