Passarelli Mantovani Rebeca, Sandri Angela, Boaretti Marzia, Grilli Alessandra, Volpi Sonia, Melotti Paola, Burlacchini Gloria, Lleò Maria M, Signoretto Caterina
Department of Diagnostics and Public Health, Microbiology Section, University of Verona, Verona, Italy.
Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata (AOUI), Verona, Italy.
J Oral Microbiol. 2019 Aug 7;11(1):1647036. doi: 10.1080/20002297.2019.1647036. eCollection 2019.
Recent findings indicate that the oral cavity acts as a bacterial reservoir and might contribute to the transmission of bacteria to the lower airways. Control of a potentially pathogenic microbiota might contribute to prevent the establishment of chronic infection in cystic fibrosis. We evaluated the presence of CF microorganisms in saliva and toothbrushes of CF patients and verify their possible transmission to lower airways. : We assessed the presence of , and yeasts in saliva, toothbrushes and sputum of 38 CF patients and assessed the clonal identity of the strains occurring contemporary in multiple sites by PFGE. : At least one of the investigated species was isolated from 60 saliva samples and 23 toothbrushes. was the most abundant species, followed by Candida spp. 31 patients contemporary had the same species in sputum and saliva/toothbrush: in most cases, clonal identity of the strains among the different sites was confirmed. : Toothbrushes may be sources of oral contamination and might act as reservoirs favoring transmission of potentially pathogenic microorganisms from the environment to the oral cavity and eventually to the LAW. Oral hygiene and toothbrush care are important strategies to prevent CF lung infections.
近期研究结果表明，口腔充当着细菌储存库的角色，可能会促使细菌传播至下呼吸道。控制潜在致病性微生物群可能有助于预防囊性纤维化患者慢性感染的发生。我们评估了囊性纤维化（CF）患者唾液和牙刷中CF微生物的存在情况，并验证它们向下呼吸道传播的可能性。我们评估了38例CF患者唾液、牙刷和痰液中细菌、酵母菌的存在情况，并通过脉冲场凝胶电泳（PFGE）评估多个部位同时出现的菌株的克隆一致性。在60份唾液样本和23把牙刷中至少分离出一种被调查的菌种。金黄色葡萄球菌是最常见的菌种，其次是念珠菌属。31例患者的痰液与唾液/牙刷中同时存在相同菌种：在大多数情况下，不同部位菌株的克隆一致性得到了证实。牙刷可能是口腔污染的来源，可能充当有利于潜在致病微生物从环境传播至口腔并最终传播至下呼吸道的储存库。口腔卫生和牙刷护理是预防CF肺部感染的重要策略。
