采用新型反转引物的 pmoA 定量 PCR 与芬兰沼泽湿地的潜在甲烷氧化作用相关。
Quantitative PCR of pmoA using a novel reverse primer correlates with potential methane oxidation in Finnish fen.
机构信息
Finnish Forest Research Institute, Vantaa Research Unit, Box 18, FI-01301 Vantaa, Finland.
出版信息
Res Microbiol. 2009 Dec;160(10):751-6. doi: 10.1016/j.resmic.2009.09.008. Epub 2009 Sep 23.
We report a new reverse primer (A621r) for use with A189f in PCR amplification of pmoA alleles in type II methanotrophs. The new primer combination was used to successfully amplify pmoA in peat monolith samples of various depths taken from fen-type peatlands in Finland. In quantitative PCR, pmoA amplicons produced from two sets of three replicate monoliths showed a significant Pearson correlation coefficient (r=0.77 and 0.61) with methane oxidation potential. The maximum methane oxidation potential and number of pmoA amplicons ranged between 8.8-40.5 micromol g (dry weight)(-1) d(-1) and 5.5 x 10(7)-18.7 x 10(7) g (wet weight)(-1), respectively, occurring in depths between 10 and 30 cm beneath the surface in the seven individual monoliths used in this study.
我们报告了一种新的反向引物（A621r），用于与 A189f 一起在 II 型甲烷营养菌的 pmoA 等位基因的 PCR 扩增中使用。新的引物组合成功地扩增了来自芬兰沼泽型泥炭地不同深度的泥炭柱样品中的 pmoA。在定量 PCR 中，来自两组三个重复柱的 pmoA 扩增子与甲烷氧化潜能显示出显著的皮尔逊相关系数（r=0.77 和 0.61）。最大甲烷氧化潜能和 pmoA 扩增子数量分别在 8.8-40.5 微摩尔 g（干重）（-1）d（-1）和 5.5 x 10（7）-18.7 x 10（7）g（湿重）（-1）之间变化，分别出现在本研究中使用的七个单独的柱中 10 到 30 厘米深的地方。
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