Department of Food Science and Technology, Division of Food Chemistry, BOKU - University of Natural Resources and Applied Life Sciences Vienna, Gregor Mendel-Strasse 33, A-1180 Vienna, Austria.
J Chromatogr A. 2010 May 7;1217(19):3251-7. doi: 10.1016/j.chroma.2009.09.027. Epub 2009 Sep 13.
A fast and reliable ultra-performance liquid chromatography (UPLC) method for the determination of biogenic amines (ethanolamine, methylamine, agmatine, histamine, dimethylamine, ethylamine, octopamine, pyrrolidine, dopamine, isopropylamine, propylamine, tyramine, putrescine, butylamine, cadaverine, tryptamine, 2-phenylethylamine, 3-methylbutylamine, spermidine, spermine) in cheese was established. After pre-column derivatization with 6-aminoquinolyl-N-hydroxy-succinimidyl carbamate (AQC), 20 primary and secondary biogenic amines were separated on an Acquity UPLC column (BEH C(18), 1.7 microm; 2.1 mm x 50 mm) within 9 min. Limits of detection (mg/100g cheese) ranged from 0.04 (ethanolamine) to 1.62 (spermine), and limits of quantification were between 0.16 (ethanolamine) and 6.09 (spermine). The UPLC method was applied to the analysis of 58 cheese samples as retailed in Austria. About 13.8% of samples had a histamine content above 10mg/100g, and 22.4% had a tyramine content above 10mg/100g. Moreover, 8.6% of samples had a putrescine or cadaverine content higher than 10mg/100g. The total concentration of biogenic amines in two cheese samples was about 194 mg/100g. Thus, obligatory monitoring of biogenic amines should be considered to ensure quality of cheese in future.
建立了奶酪中生物胺（乙醇胺、甲胺、胍丁胺、组胺、二甲胺、乙胺、章鱼胺、吡咯烷、多巴胺、异丙胺、丙胺、酪胺、腐胺、丁胺、尸胺、色胺、2-苯乙胺、3-甲基丁胺、亚精胺、精胺）的快速可靠的超高效液相色谱（UPLC）测定方法。经 6-氨基喹啉基-N-羟基琥珀酰亚胺基碳酸酯（AQC）柱前衍生后，20 种伯仲生物胺在 Acquity UPLC 柱（BEH C(18)，1.7 µm；2.1 mm x 50 mm）上可在 9 min 内分离。检测限（mg/100g 奶酪）范围为 0.04（乙醇胺）至 1.62（精胺），定量限在 0.16（乙醇胺）至 6.09（精胺）之间。该 UPLC 方法应用于分析奥地利零售的 58 种奶酪样品。约 13.8%的样品组胺含量高于 10mg/100g，22.4%的样品酪胺含量高于 10mg/100g。此外，8.6%的样品腐胺或尸胺含量高于 10mg/100g。两种奶酪样品中生物胺的总浓度约为 194 mg/100g。因此，今后应考虑对生物胺进行强制性监测，以确保奶酪的质量。
