Hernández-Borges Javier, D'Orazio Giovanni, Aturki Zeineb, Fanali Salvatore
Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, P.O. Box 10, Area della Ricerca di Roma, Monterotondo Scalo, Rome, Italy.
J Chromatogr A. 2007 Apr 20;1147(2):192-9. doi: 10.1016/j.chroma.2007.02.072. Epub 2007 Feb 24.
In this work, the simultaneous analysis of 10 biogenic amines (ethanolamine, methylamine, tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine) in wines by nano-liquid chromatography (nano-LC) using UV detection and a capillary bidentate C(18) column of 100 microm I.D. is proposed. The 10 selected amines, which are the most important to be determined in wine samples, were derivatized with dansyl-chloride (Dns-Cl) previous to their nano-LC determination. Excess of the derivatizing agent as well as other components of the samples were eliminated by the use of an on-line cleaning step employing a C(18) trapping column which also provided a pre-concentration effect. The mobile phase composed of acetonitrile, water, acetic acid and triethylamine (TEA) mixture was pumped at a low flow rate (634 nL/min). Limits of detection (LODs) achieved ranged between 18.3 and 48.3 ng/mL; while calibration curves showed good linearity (R(2)>0.9924). The method was applied to the analysis of this group of amines in white and red wine samples after suitable treatment with polyvinylpyrrolidone (PVP) and extraction with C(18) cartridges.
本文提出了一种采用紫外检测和内径为100微米的毛细管双齿C(18)柱，通过纳升液相色谱(nano-LC)同时分析葡萄酒中10种生物胺(乙醇胺、甲胺、色胺、2-苯乙胺、腐胺、尸胺、组胺、酪胺、亚精胺和精胺)的方法。所选择的这10种胺是葡萄酒样品中最重要的待测定胺类，在进行纳升液相色谱测定之前，先用丹磺酰氯(Dns-Cl)对其进行衍生化。通过使用C(18)捕集柱的在线清洗步骤消除过量的衍生化试剂以及样品中的其他成分，该步骤还提供了预浓缩效果。由乙腈、水、乙酸和三乙胺(TEA)混合物组成的流动相以低流速(634纳升/分钟)泵送。检测限(LOD)在18.3至48.3纳克/毫升之间；校准曲线显示出良好的线性(R(2)>0.9924)。在用聚乙烯吡咯烷酮(PVP)进行适当处理并用C(18)柱萃取后，该方法应用于白葡萄酒和红葡萄酒样品中这组胺类的分析。
