Laboratoire Matière et Systèmes Complexes, UMR CNRS 7057, University Paris-Diderot, Bâtiment Condorcet, Case 7056, F-75205 Paris Cedex 13, France.
Biomaterials. 2009 Dec;30(36):6920-8. doi: 10.1016/j.biomaterials.2009.08.056. Epub 2009 Sep 24.
Autografting of cultured myogenic precursor cells (MPC) is a therapeutic strategy for muscle disorders, including striated urethral sphincter insufficiency. Implantation of myofibers with their satellite cells into the urethra is a recently described method of MPC transfer aimed at generating a new sphincter in incontinent patients. In this study, we magnetically labeled muscle implants with dextran-free anionic iron oxide nanoparticles (AMNP). The aim was to evaluate the biocompatibility of the labeling procedure and its utility for non-invasive MRI follow-up of cell therapy in a female pig model. After adsorption of AMNP to the implant surface, various cell types, including MPC, were magnetically labeled within the implants. Magnetic labeling did not affect cell proliferation or differentiation. Autograft detection in vivo by 0.3-T MRI was possible for up to 1 month. Ex vivo, Perl's, anti-desmin and anti-myosin heavy chain staining confirmed the co-localization of AMNP and regenerated myofibers. AMNP labeling was thus useful for locating myofiber implant autografts in vivo and for ex vivo monitoring of the biology of this cell transfer method.
自体移植培养的肌源性前体细胞（MPC）是一种治疗肌肉疾病的策略，包括横纹尿道括约肌功能不全。将带有卫星细胞的肌纤维植入尿道是一种最近描述的 MPC 转移方法，旨在为失禁患者产生新的括约肌。在这项研究中，我们使用无右旋糖酐的阴离子氧化铁纳米颗粒（AMNP）对肌肉植入物进行磁标记。目的是评估标记程序的生物相容性及其在女性猪模型中用于细胞治疗的非侵入性 MRI 随访的效用。在植入物表面吸附 AMNP 后，包括 MPC 在内的各种细胞类型都可以在植入物内进行磁性标记。磁性标记不会影响细胞增殖或分化。通过 0.3-T MRI 可以在体内检测到自体移植物长达 1 个月。离体时，Perl's、抗结蛋白和抗肌球蛋白重链染色证实了 AMNP 和再生肌纤维的共定位。因此，AMNP 标记可用于在体内定位肌纤维植入物自体移植物，并用于体外监测这种细胞转移方法的生物学特性。
