Wilhelm Claire, Gazeau Florence
Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057 CNRS et Université Paris-Diderot, Paris, France.
Biomaterials. 2008 Aug;29(22):3161-74. doi: 10.1016/j.biomaterials.2008.04.016. Epub 2008 May 1.
Magnetic labelling of living cells creates opportunities for numerous biomedical applications, from individual cell manipulation to MRI tracking. Here we describe a non-specific labelling method based on anionic magnetic nanoparticles (AMNPs). These particles first adsorb electrostatically to the outer membrane before being internalized within endosomes. We compared the labelling mechanism, uptake efficiency and biocompatibility with 14 different cell types, including adult cells, progenitor cells, immune cells and tumour cells. A single model was found to describe cell/nanoparticle interactions and to predict uptake efficiency by all the cell types. The potential impact of the AMNP label on cell functions, in vitro and in vivo, is discussed according to cellular specificities. We also show that the same label provides sufficient magnetization for MRI detection and distal manipulation.
活细胞的磁性标记为众多生物医学应用创造了机会，从单个细胞操作到磁共振成像（MRI）追踪。在此，我们描述了一种基于阴离子磁性纳米颗粒（AMNP）的非特异性标记方法。这些颗粒首先通过静电吸附到外膜上，然后被内吞到内体中。我们比较了14种不同细胞类型（包括成体细胞、祖细胞、免疫细胞和肿瘤细胞）的标记机制、摄取效率和生物相容性。发现一个单一模型可描述细胞/纳米颗粒相互作用，并预测所有细胞类型的摄取效率。根据细胞特异性讨论了AMNP标记在体外和体内对细胞功能的潜在影响。我们还表明，相同的标记为MRI检测和远程操作提供了足够的磁化强度。
