从不含多糖干扰的载细胞水凝胶支架中同时提取蛋白质和RNA。
Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference.
作者信息
Wang Chunming, Li Xin, Yao Yongchang, Wang Dong-An
机构信息
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 1;877(29):3762-6. doi: 10.1016/j.jchromb.2009.09.012. Epub 2009 Sep 17.
Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.
从含细胞支架中同时提取蛋白质和RNA对生物材料学家和组织工程师将有很大帮助。在此，我们描述了一种从富含多糖的水凝胶支架中分离RNA过程中产生的废弃溶液中提取蛋白质的方法。这种方法能够从同一样本中获取蛋白质和RNA，同时消除多糖干扰。
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