Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Minami, Daiko, Higashi-ku, Nagoya City, Aichi, Japan.
Exp Mol Pathol. 2010 Feb;88(1):90-5. doi: 10.1016/j.yexmp.2009.09.010. Epub 2009 Sep 24.
Rho family protein regulates variety of cellular functions as cytoskeletal organization, cell proliferation and apoptosis. In the present study, we demonstrate that RhoB-overexpressed prostate cancer cells showed an enhanced cell motility and the administration of the GSK-3 inhibitors inhibited this increase in migration. Among the extracellular matrix and adhesion-related molecules, MMP1 RNA expression was increased in RhoB-overexpressed cells, administration of MMP inhibitor suppressed the collagen gel invasion in these cells. This is the first report evaluating RhoB function and the downstream signaling events in prostate cancer cell. Our results indicate that RhoB promotes cell motility and invasion in a metastatic prostate cancer cell.
Rho 家族蛋白调节多种细胞功能，如细胞骨架组织、细胞增殖和细胞凋亡。在本研究中，我们证明 RhoB 过表达的前列腺癌细胞表现出增强的细胞迁移能力，而 GSK-3 抑制剂的给药抑制了这种迁移增加。在细胞外基质和粘附相关分子中，RhoB 过表达细胞中 MMP1 RNA 的表达增加，MMP 抑制剂的给药抑制了这些细胞在胶原凝胶中的侵袭。这是评估 RhoB 在前列腺癌细胞中的功能和下游信号事件的第一项报告。我们的结果表明，RhoB 促进转移性前列腺癌细胞的运动和侵袭。
