Cole Karen C, Barbour Joy-El R, Midkiff John F, Marble Brittany M, Johnson Douglas I
Department of Microbiology and Molecular Genetics, University of Vermont, 202 Stafford Hall, 95 Carrigan Drive, VT 05405, United States.
FEBS Lett. 2009 Oct 20;583(20):3339-43. doi: 10.1016/j.febslet.2009.09.033. Epub 2009 Sep 24.
Targeting of Saccharomyces cerevisiae Cdc24p to polarized growth sites is essential for its function. Localization of GFP-tagged Cdc24 proteins or fragments was assayed in deletion mutants of Cdc24p-interacting proteins. The boi2Delta, ent2Delta, and hua1Delta mutants showed localization defects. The tos2Delta skg6Delta double mutant displayed aberrant pre-anaphase localization to the mother-bud neck region. The same aberrant pattern was seen when potential phosphorylation sites Ser697, Thr704, and Tyr200 were mutated. The S697A mutation also resulted in phosphorylation defects in vivo. These data support roles for Boi2p, Ent2p, Hua1p, Tos2p, and for Cdc24p phosphorylation in targeting Cdc24p to growth sites.
将酿酒酵母Cdc24p靶向极化生长位点对其功能至关重要。在与Cdc24p相互作用蛋白的缺失突变体中检测了GFP标记的Cdc24蛋白或片段的定位。boi2Δ、ent2Δ和hua1Δ突变体显示出定位缺陷。tos2Δskg6Δ双突变体在前期前异常定位于母-芽颈部区域。当潜在的磷酸化位点Ser697、Thr704和Tyr200发生突变时，观察到相同的异常模式。S697A突变在体内也导致磷酸化缺陷。这些数据支持Boi2p、Ent2p、Hua1p、Tos2p以及Cdc24p磷酸化在将Cdc24p靶向生长位点中的作用。
