REQUIMTE-Departmento de Química, Faculdade de Ciencias e Tecnologia (UNL), 2829-516 Monte Caparica, Portugal.
Sensors (Basel). 2010;10(12):11530-55. doi: 10.3390/s101211530. Epub 2010 Dec 15.
The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although several analytical protocols have been proposed for nitrite quantification, none of them enable a reliable and quick analysis of complex samples. An alternative approach relies on the construction of biosensing devices using stable enzymes, with both high activity and specificity for nitrite. In this paper we review the current state-of-the-art in the field of electrochemical and optical biosensors using nitrite reducing enzymes as biorecognition elements and discuss the opportunities and challenges in this emerging market.
过去几十年，社会和政治意识不断增强，人们需要对环境和工业过程进行监测和控制。就亚硝酸盐离子而言，由于其对人类健康具有潜在毒性，欧盟最近出台了多项规定来限制饮用水和食品中亚硝酸盐离子的含量。尽管已经提出了几种用于亚硝酸盐定量分析的分析方案，但没有一种方案能够对复杂样品进行可靠和快速的分析。另一种方法是使用稳定的酶来构建生物传感装置，这些酶对亚硝酸盐具有高活性和特异性。本文综述了电化学和光学生物传感器领域的最新进展，这些生物传感器使用亚硝酸盐还原酶作为生物识别元件，并讨论了这一新兴市场的机遇和挑战。
