Universitary Institute IVI Valencia, Instituto Valenciano de Infertilidad, Plaza Policía Local, 3, Valencia 46015, Spain.
Fertil Steril. 2010 Feb;93(2):669-71. doi: 10.1016/j.fertnstert.2009.08.041. Epub 2009 Sep 26.
This article describes a new methodology for preserving and banking isolated human blastomeres, whose originality is based on packing the blastomere into an emptied zona pellucida before vitrification. After warming, 75.7% of blastomeres survived and developed at a rate comparable to that in noncryopreserved blastomeres (62.5% cleavage, 26.6% compaction, and 20.3% cavitation).
本文描述了一种保存和储存分离的人类卵裂球的新方法，其创新性在于在玻璃化之前将卵裂球装入已排空的透明带内。解冻后，75.7%的卵裂球存活并发育，其速度与非冷冻卵裂球相当（62.5%卵裂，26.6%致密化和 20.3%空化）。
