De Cássia Savio Figueira Rita, Souza Setti Amanda, Paes De Almeida Ferreira Braga Daniela, Iaconelli Assumpto, Borges Edson
Fertility - Assisted Fertilization Center, São Paulo, SP, Brazil.
Hum Fertil (Camb). 2010 Sep;13(3):143-50. doi: 10.3109/14647273.2010.508505.
The aim of the study was to discover which intracytoplasmic sperm injection (ICSI) cycle parameters could influence the presence of multinucleated blastomeres (MNBs) and how ICSI outcomes are influenced by this event.
Embryos derived from normally fertilised oocytes were divided into two groups: embryos that had only mononucleated blastomeres (NBs group n = 2818) and embryos that had at least one multinucleated blastomere (MNB group, n = 404). The effects of ICSI cycle factors on multinucleation were investigated and embryo development was compared between the groups. The cycles were also split into those in which only NB embryos were present (NB cycles, n = 298) and cycles in which MNB embryos were present (MNB cycles, n = 203). ICSI outcomes were compared between the groups.
A higher incidence of MNB embryos arose in pituitary blockage with gonadotropin-releasing hormone agonists, male factor infertility and in cycles with higher number of retrieved oocytes. Embryos that had only one affected blastomere showed greater development than embryos with more than one affected blastomere. Finally, the implantation rate decreased when MNB embryos were transferred.
Multinucleation events may be affected by aspects of the ICSI cycle and compromise embryo quality and implantation rate.
本研究的目的是发现哪些胞浆内单精子注射（ICSI）周期参数会影响多核卵裂球（MNBs）的出现，以及这一事件如何影响ICSI的结果。
将正常受精的卵母细胞来源的胚胎分为两组：只有单核卵裂球的胚胎（NB组，n = 2818）和至少有一个多核卵裂球的胚胎（MNB组，n = 404）。研究了ICSI周期因素对多核化的影响，并比较了两组胚胎的发育情况。这些周期还被分为仅存在NB胚胎的周期（NB周期，n = 298）和存在MNB胚胎的周期（MNB周期，n = 203）。比较了两组的ICSI结果。
在使用促性腺激素释放激素激动剂进行垂体阻滞、男性因素不育以及回收卵母细胞数量较多的周期中，MNB胚胎的发生率较高。只有一个受影响卵裂球的胚胎比有一个以上受影响卵裂球的胚胎发育更好。最后，移植MNB胚胎时着床率会降低。
多核化事件可能受ICSI周期各方面的影响，并会损害胚胎质量和着床率。
