Chemical Engineering and Environmental Engineering Program, Yale University, New Haven, CT 06513, USA.
Environ Pollut. 2010 Feb;158(2):470-5. doi: 10.1016/j.envpol.2009.08.022. Epub 2009 Sep 25.
Phosphor imager autoradiography is a technique for rapid, sensitive analysis of the localization of xenobiotics in plant tissues. Use of this technique is relatively new to research in the field of plant science, and the potential for enhancing visualization and understanding of plant uptake and transport of xenobiotics remains largely untapped. Phosphor imager autoradiography is used to investigate the uptake and translocation of the explosives 1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene within Populus deltoides x nigra DN34 (poplar) and Panicum vigratum Alamo (switchgrass). In both plant types, TNT and/or TNT-metabolites remain predominantly in root tissues while RDX and/or RDX-metabolites are readily translocated to leaf tissues. Phosphor imager autoradiography is further investigated for use in semi-quantitative analysis of uptake of TNT by switchgrass.
荧光成像放射自显影是一种快速、灵敏的分析技术，用于研究植物组织中外来化合物的定位。这项技术在植物科学研究中相对较新，其增强外来化合物在植物体内的吸收和运输可视化和理解的潜力在很大程度上尚未开发。荧光成像放射自显影用于研究爆炸物 1,3,5-三硝基-1,3,5-三嗪（RDX）和 2,4,6-三硝基甲苯在杂种白杨（Populus deltoides x nigra DN34，杨树）和柳枝稷（Panicum vigratum Alamo，柳枝稷）中的吸收和转运。在这两种植物类型中，TNT 和/或 TNT 代谢物主要仍存在于根组织中，而 RDX 和/或 RDX 代谢物则容易转运到叶片组织中。荧光成像放射自显影进一步用于半定量分析柳枝稷对 TNT 的吸收。
