Saji M, Miura M
Department of Physiology, Gunma University School of Medicine, Maebashi, Japan.
Neurosci Lett. 1990 Jul 31;115(2-3):177-82. doi: 10.1016/0304-3940(90)90451-e.
Glutamate immunoreactivity was found in 81-84% of the bulbospinal neurons projecting to the phrenic motoneuron pool, which were located in the para-ambiguous nucleus and the ventral vicinity of the retrofacial nucleus. Their glutamate contents were identified by a double labeling technique in combination with the retrograde transport of horseradish peroxidase and immunocytochemistry of the glutamate. The remaining 16-19% of the bulbospinal neurons projecting to the phrenic motoneuron pool did not contain glutamate immunoreactivity. Thus it appears that glutamate may be a major transmitter of the inspiratory drive from the medullary respiratory neurons to the phrenic motoneurons.
在投射至膈运动神经元池的延髓脊髓神经元中，81%-84%可检测到谷氨酸免疫反应性，这些神经元位于疑核和迷走神经后核腹侧附近。通过结合辣根过氧化物酶逆行运输和谷氨酸免疫细胞化学的双重标记技术，确定了它们的谷氨酸含量。其余16%-19%投射至膈运动神经元池的延髓脊髓神经元不含有谷氨酸免疫反应性。因此，谷氨酸似乎可能是从延髓呼吸神经元到膈运动神经元的吸气驱动的主要递质。
