Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy.
J Clin Virol. 2009 Dec;46(4):367-70. doi: 10.1016/j.jcv.2009.09.011. Epub 2009 Sep 25.
Recent data have shown that plasma levels of lipopolysaccharide (LPS) are a quantitative indicator of microbial translocation in HIV infected individuals.
To assess the impact of residual viral replication on plasma LPS in HAART-treated HIV+ subjects with <50copies/ml HIV-1 RNA and to evaluate LPS changes during repeated HAART interruptions not exceeding 2-month duration.
LPS was measured in 44 HIV+ subjects at T0 (during HAART) and at day 15 of the first and fourth HAART interruption. Ten uninfected, healthy donors were studied as well. Residual plasma HIV-1 RNA was measured at T0 by an ultra-ultrasensitive method with limit of detection of 2.5copies HIV-1 RNA/ml. Subjects with less than 2.5copies/ml (fully suppressed - FS) were compared to those with 2.5-50copies/ml (partially suppressed - PS).
At T0, plasma LPS levels were comparable in FS and uninfected subjects, whereas in PS they were higher than in uninfected subjects (p=0.049). After 4 HAART interruptions, they did not change significantly. However, LPS values were lower in FS than in PS (p=0.020). An inverse correlation was found between CD4 and LPS levels (p=0.044) in PS group only.
A reduced degree of microbial translocation was seen in subjects with a more complete suppression of viral replication. Repeated HAART interruptions had no significant impact on plasma LPS levels.
最近的数据表明，脂多糖（LPS）的血浆水平是 HIV 感染者微生物易位的定量指标。
评估在 HIV-1 RNA<50 拷贝/ml 的接受 HAART 治疗的 HIV+患者中，残余病毒复制对血浆 LPS 的影响，并评估不超过 2 个月持续时间的重复 HAART 中断期间 LPS 的变化。
在 T0（HAART 期间）以及第一次和第四次 HAART 中断的第 15 天，对 44 名 HIV+患者测量 LPS。还研究了 10 名未感染的健康供体。在 T0 时通过超灵敏方法测量残余血浆 HIV-1 RNA，检测限为 2.5 拷贝 HIV-1 RNA/ml。将 HIV-1 RNA<2.5 拷贝/ml（完全抑制 - FS）的患者与 2.5-50 拷贝/ml（部分抑制 - PS）的患者进行比较。
在 T0，FS 和未感染患者的血浆 LPS 水平相当，而 PS 患者的血浆 LPS 水平高于未感染患者（p=0.049）。经过 4 次 HAART 中断后，它们没有显著变化。然而，FS 的 LPS 值低于 PS（p=0.020）。仅在 PS 组中发现 CD4 和 LPS 水平之间存在负相关（p=0.044）。
在病毒复制抑制程度更高的患者中，观察到微生物易位程度降低。重复 HAART 中断对血浆 LPS 水平没有显著影响。
