Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
Dev Comp Immunol. 2010 Mar;34(3):250-7. doi: 10.1016/j.dci.2009.09.006. Epub 2009 Oct 8.
The swine leukocyte antigen (SLA) haplotype B is associated with increased penetrance of the tumor traits in Sinclair swine cutaneous melanoma (SSCM). We established a series of SinclairxHanford swine crosses to facilitate genetic mapping of the tumor-associated loci. In this study, the SLA diversity in the founding animals was characterized for effective selection of maximum tumor penetrance in the pedigrees. Using the sequence-based typing (SBT) method we identified a total of 29 alleles at five polymorphic SLA loci (SLA-1, SLA-3, SLA-2, DRB1 and DQB1) representing six class I and five class II haplotypes. We subsequently developed a rapid PCR-based typing assay using sequence-specific primers (PCR-SSP) to efficiently follow the SLA types of the crossbred progeny. In a total of 469 animals we identified three crossovers within the class I region and three between the class I and class II regions, which corresponded to recombination frequencies of 0.39% and 0.56%, respectively. We also confirmed the presence of two expressed SLA-1 loci in three of the class I haplotypes and were able to determine the relative chromosomal arrangement of the duplicated loci in two haplotypes. This study furthers our understanding of the allelic architecture and polymorphism of the SLA system and will facilitate the mapping of loci associated with the expression of SSCM.
猪白细胞抗原（SLA）单体型 B 与 Sinclair 猪皮肤黑色素瘤（SSCM）肿瘤特征的外显率增加有关。我们建立了一系列 SinclairxHanford 猪杂交，以促进与肿瘤相关基因座的遗传作图。在这项研究中，对创始动物的 SLA 多样性进行了特征描述，以便在系谱中有效选择最大的肿瘤穿透性。使用基于序列的分型（SBT）方法，我们在五个多态性 SLA 基因座（SLA-1、SLA-3、SLA-2、DRB1 和 DQB1）中总共鉴定了 29 个等位基因，代表六个 I 类和五个 II 类单体型。随后，我们使用序列特异性引物（PCR-SSP）开发了一种快速的基于 PCR 的分型检测方法，以有效地跟踪杂交后代的 SLA 类型。在总共 469 只动物中，我们在 I 类区域内鉴定了三个交叉，在 I 类和 II 类区域之间鉴定了三个交叉，对应的重组频率分别为 0.39%和 0.56%。我们还证实了三个 I 类单体型中存在两个表达的 SLA-1 基因座，并能够确定两个单体型中重复基因座的相对染色体排列。这项研究进一步了解了 SLA 系统的等位基因结构和多态性，并将有助于与 SSCM 表达相关的基因座的定位。
