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糖甜菜无融合生殖单体附加系 M14 的蛋白质组学分析。

Proteomic analysis of sugar beet apomictic monosomic addition line M14.

机构信息

Heilongjiang University, Harbin, China.

出版信息

J Proteomics. 2009 Dec 1;73(2):297-308. doi: 10.1016/j.jprot.2009.09.012. Epub 2009 Sep 25.

Abstract

Apomixis in plants holds great promise for agriculture because of its vigor associated with heterozygosity and superior genotype. Despite the significance of apomictic reproductive process, our knowledge of proteins and their functions in apomictic development is limited. Here we report a comparative proteomic and transcriptomic analysis of sexual and apomictic processes in sugar beet. A total of 71 differentially expressed protein spots were successfully identified in the course of apomictic reproductive development using high-resolution 2-DE and MS analysis. The differentially expressed proteins were involved in several processes that might work cooperatively to lead to apomictic reproduction. This study has generated potential protein markers important for apomictic development.

摘要

植物中的无融合生殖因其与杂合性和优良基因型相关的活力而在农业中具有巨大的应用前景。尽管无融合生殖过程意义重大,但我们对其蛋白质及其在无融合生殖发育中的功能的了解是有限的。在这里,我们报告了甜菜有性和无融合生殖过程的比较蛋白质组学和转录组学分析。通过高分辨率 2-DE 和 MS 分析,在无融合生殖发育过程中成功鉴定了总共 71 个差异表达的蛋白质斑点。差异表达的蛋白质参与了几个可能协同作用以导致无融合生殖的过程。这项研究产生了对无融合生殖发育很重要的潜在蛋白质标记物。

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