College of Life Sciences, Heilongjiang University, Harbin 150080, China.
J Plant Physiol. 2011 Nov 1;168(16):1980-6. doi: 10.1016/j.jplph.2011.05.027. Epub 2011 Jul 31.
Monosomic addition line M14 carrying an additional chromosome 9 from Beta corolliflora Zosimovic ex Buttler was obtained through hybridization between the wild species B. corolliflora and a cultivated species Beta vulgaris L. var Saccharifera Alef. The M14 line showed diplosporic reproduction and stress tolerance. To identify differentially expressed genes in M14, a subtractive cDNA library was prepared by suppression subtractive hybridization (SSH) between M14 (2n=18+1) and B. vulgaris (2n=18). A total of 190 unique sequences were identified in the library and their putative functions were analyzed using Gene Ontology (GO). One of the genes, designated as BvM14-MADS box, encodes a MADS box transcription factor. It was cloned from M14 and over-expressed in transgenic tobacco plants. Interestingly, this gene was located on chromosome 2 of B. vulgaris, not on the additional chromosome 9. Overexpression of BvM14-MADS box led to significant phenotypic changes in tobacco. The differential expression of BvM14-MADS box gene in M14 may be caused by the interaction between the additional chromosome 9 from B. corolliflora and the B. vulgaris chromosomes in M14.
单体附加系 M14 是通过野生种 Beta corolliflora Zosimovic ex Buttler 与栽培种 Beta vulgaris L. var Saccharifera Alef 杂交获得的，它携带了来自 Beta corolliflora 的额外的第 9 号染色体。M14 系表现出二倍体生殖和应激耐受。为了鉴定 M14 中差异表达的基因，我们通过抑制性消减杂交（SSH）在 M14（2n=18+1）和 Beta vulgaris（2n=18）之间制备了一个消减 cDNA 文库。文库中鉴定出了 190 个独特的序列，并使用基因本体论（GO）分析了它们的可能功能。其中一个基因，命名为 BvM14-MADS box，编码一个 MADS 框转录因子。它是从 M14 中克隆出来的，并在转基因烟草植物中过表达。有趣的是，这个基因位于 Beta vulgaris 的第 2 号染色体上，而不是在额外的第 9 号染色体上。BvM14-MADS box 的过表达导致烟草出现显著的表型变化。M14 中 BvM14-MADS box 基因的差异表达可能是由来自 Beta corolliflora 的额外的第 9 号染色体与 M14 中的 Beta vulgaris 染色体之间的相互作用引起的。
