A novel cobalt hexacyanoferrate nanocomposite on CNT scaffold by seed medium and application for biosensor.

In this paper, for the first time, we introduced the seed-mediated method to the growth of cobalt hexacyanoferrate nanoparticles (CoNPs), using 3.5 nm gold nanoparticles as seeds and multiwalled carbon nanotubes (MWCNTs) as growth scaffold which would both show synergistic action toward the reduction of H2O2. Via gold seeds, the one-step fabrication of CoNPs on the glassy carbon electrode is simple without any linking reagents, which will ingeniously exert the electrochemical properties of cobalt hexacyanoferrate. Combined with glucose oxidase, the sensing surface is applied as a biosensor for glucose. The growth of CoNPs is a chemical deposition process around the small Au nanoseed particles. The nanoseeds bridge the CoNPs and CNTs to form a smart nanocomposite. Spherical CoNPs have a relatively moderate dispersion on the three-dimensional network of CNTs with relatively even diameter ca. 100 nm. Whereas, in the control experiments without gold seeds cobalt hexacyanoferrate can only form continuous films, of which the size is far from nanolevel and the catalytic ability is poor. The synthesis and fabrication/modification of CoNPs are simple and fast without prior preparation of CoNPs and lengthy process of cross-linking. The amount of the seeds and CNTs, growth time and concentration of growth solution were investigated. Scanning electron microscopy (SEM) and electrochemical method were used.