The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
College of Acumox and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
Biosens Bioelectron. 2014 Dec 15;62:73-8. doi: 10.1016/j.bios.2014.06.030. Epub 2014 Jun 20.
A facile approach was developed for the preparation of nanocomposite based on β-lactoglobulin (BLG)-functionalized multi-wall carbon nanotubes (MWCNTs) and gold nanoparticles (GNPs) for the first time. Owing to the amphipathic nature, BLG can be adopted onto the surface of MWCNTs to form BLG-MWCNTs with uniform dispersion in water. Taking advantage of sulfhydryl groups on BLG-MWCNTs, GNPs were decorated on the BLG-MWCNTs-modified glassy carbon electrode (GCE) by electrodeposition. The nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy and X-ray spectroscopy analysis. Cyclic voltammetry and chronoamperometric method were used to evaluate the electrocatalytic ability of the nanocomposite. Furthermore, a glucose biosensor was developed based on the immobilization of glucose oxidase with cross-linking in the matrix of bovine serum albumin (BSA) on the nanocomposite modified GCE. The resulting biosensor exhibited high sensitivity (3.98 μA mM(-1)), wider linear range (0.025-5.5 mM), low detection limit (1.1 μM at the signal-to-noise ratio of 3) and fast response time (within 7s) for glucose detection.
首次开发了一种基于β-乳球蛋白（BLG）功能化多壁碳纳米管（MWCNTs）和金纳米粒子（GNPs）的纳米复合材料的制备方法。由于两亲性，BLG 可以被吸附到 MWCNTs 的表面上，从而在水中形成均匀分散的 BLG-MWCNTs。利用 BLG-MWCNTs 上的巯基，通过电沉积将 GNPs 修饰在 BLG-MWCNTs 修饰的玻碳电极（GCE）上。通过透射电子显微镜、扫描电子显微镜和 X 射线能谱分析对纳米复合材料进行了表征。循环伏安法和计时电流法用于评估纳米复合材料的电催化能力。此外，基于牛血清白蛋白（BSA）基质中交联固定葡萄糖氧化酶，在纳米复合材料修饰的 GCE 上开发了葡萄糖生物传感器。所得生物传感器对葡萄糖检测表现出高灵敏度（3.98 μA mM(-1)）、较宽的线性范围（0.025-5.5 mM）、低检测限（信噪比为 3 时为 1.1 μM）和快速响应时间（7s 内）。
