Tibourtine Fahd, Canceill Thibault, Marfoglia Andrea, Lavalle Philippe, Gibot Laure, Pilloux Ludovic, Aubry Clementine, Medemblik Claire, Goudouneche Dominique, Dupret-Bories Agnès, Cazalbou Sophie
CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, CEDEX 9, 31062 Toulouse, France.
Département Odontologie, Faculté de Santé, Hôpitaux de Toulouse, Université Paul Sabatier, 3 Chemin des Maraichers, CEDEX 9, 31062 Toulouse, France.
J Funct Biomater. 2024 Feb 19;15(2):49. doi: 10.3390/jfb15020049.
Human platelet lysate (HPL), rich in growth factors, is increasingly recognized for its potential in tissue engineering and regenerative medicine. However, its use in liquid or gel form is constrained by limited stability and handling difficulties. This study aimed to develop dry and porous aerogels from HPL hydrogel using an environmentally friendly supercritical CO-based shaping process, specifically tailored for tissue engineering applications. The aerogels produced retained their three-dimensional structure and demonstrated significant mechanical robustness and enhanced manageability. Impressively, they exhibited high water absorption capacity, absorbing 87% of their weight in water within 120 min. Furthermore, the growth factors released by these aerogels showed a sustained and favourable biological response in vitro. They maintained the cellular metabolic activity of fibroblasts (BALB-3T3) at levels akin to conventional culture conditions, even after prolonged storage, and facilitated the migration of human umbilical vein endothelial cells (HUVECs). Additionally, the aerogels themselves supported the adhesion and proliferation of murine fibroblasts (BALB-3T3). Beyond serving as excellent matrices for cell culture, these aerogels function as efficient systems for the delivery of growth factors. Their multifunctional capabilities position them as promising candidates for various tissue regeneration strategies. Importantly, the developed aerogels can be stored conveniently and are considered ready to use, enhancing their practicality and applicability in regenerative medicine.
富含生长因子的人血小板裂解液（HPL）在组织工程和再生医学中的潜力日益受到认可。然而，其液体或凝胶形式的使用受到稳定性有限和操作困难的限制。本研究旨在利用一种专门为组织工程应用量身定制的环保型超临界CO基成型工艺，从HPL水凝胶制备干燥且多孔的气凝胶。所制备的气凝胶保留了其三维结构，并表现出显著的机械强度和更高的可操作性。令人印象深刻的是，它们具有高吸水能力，在120分钟内可吸收其重量87%的水分。此外，这些气凝胶释放的生长因子在体外显示出持续且良好的生物学反应。即使经过长时间储存，它们仍能将成纤维细胞（BALB-3T3）的细胞代谢活性维持在与传统培养条件相近的水平，并促进人脐静脉内皮细胞（HUVECs）的迁移。此外，气凝胶本身支持小鼠成纤维细胞（BALB-3T3）的黏附和增殖。除了作为细胞培养的优良基质外，这些气凝胶还作为生长因子递送的有效系统发挥作用。它们的多功能特性使其成为各种组织再生策略的有希望的候选者。重要的是，所开发的气凝胶可以方便地储存且被认为可随时使用，增强了它们在再生医学中的实用性和适用性。
