INRA, UR1115 Plantes et Systèmes Horticoles, Domaine Saint Paul, 84914 Avignon Cedex 09, France.
Environ Pollut. 2010 Feb;158(2):388-93. doi: 10.1016/j.envpol.2009.09.003. Epub 2009 Sep 23.
There is currently a lack of ecotoxicity tests adapted to earthworm species of higher ecological relevance and whose endpoints could be directly related to their ecological role in the soil. We propose a new and relatively simple ecotoxicity test based on the estimation of cast production (CP) by Lumbricus terrestris under laboratory conditions. CP was found to be linearly correlated to earthworm biomass and to be greatly influenced by soil water content. Azinphos-methyl had no effect on CP at all the concentrations tested. Significant decreases were observed at the normal application rate for other pesticides with (imidacloprid, carbaryl, methomyl) or without (ethyl-parathion and chlorpyrifos-ethyl) a clear concentration-effect response. For the highest concentration tested, reduction in CP varied between 35 and 67%. CP is straightforward and rapidly measured and ecologically meaningful. We thus believe it to be of great use as an endpoint in ecotoxicity testing.
目前，缺乏适应于具有更高生态相关性的蚯蚓物种的生态毒性测试，而且其终点可能与它们在土壤中的生态角色直接相关。我们提出了一种新的相对简单的生态毒性测试方法，该方法基于实验室条件下对赤子爱胜蚓的排粪量（CP）进行估算。CP 与蚯蚓生物量呈线性相关，并且受土壤含水量的影响很大。在测试的所有浓度下，甲拌磷对 CP 均没有影响。在正常施用量下，其他具有（吡虫啉、西维因、灭多威）或没有（乙基对硫磷和毒死蜱）明显浓度-效应关系的农药会导致 CP 显著减少。对于测试的最高浓度，CP 的减少量在 35%至 67%之间变化。CP 测量简单、快速，且具有生态意义。因此，我们认为它可以作为生态毒性测试中的一个重要终点。
