Svendsen C, Weeks J M
Institute of Biology, Odense University, Denmark.
Ecotoxicol Environ Saf. 1997 Feb;36(1):72-9. doi: 10.1006/eesa.1996.1491.
A simple earthworm biomarker, neutral-red retention by coelomocyte lysosomes, was measured concurrently with ecological parameters in order to link effects at different levels of biological organization in a laboratory study. Exposure of the earthworm Eisenia andrei to an increasing range of soil copper concentrations in the laboratory indicated a threshold range for the neutral-red assay at soil copper concentrations between 40 and 80 mg Cu kg-1. This threshold coincided with the soil to worm copper bioconcentration factor decreasing from 1 to 0.3 and thus an apparent onset of a copper regulatory mechanism. Effects at the individual and population levels only occurred at soil copper concentrations beyond that of the biomarker threshold. Thus, it was possible to differentiate between exposure and toxicity. It was therefore possible to conclude that the neutral-red assay has great potential and relevance in earthworm ecotoxicity studies. The assay provides an early warning that can be linked directly to important physiological changes that were observed to precede adverse effects on individuals or populations in this laboratory study.
在一项实验室研究中，为了将不同生物组织水平的效应联系起来，同时测量了一种简单的蚯蚓生物标志物——体腔细胞溶酶体对中性红的保留情况以及生态参数。在实验室中，将安德爱胜蚓暴露于土壤铜浓度不断增加的环境中，结果表明，中性红测定法的阈值范围为土壤铜浓度在40至80毫克铜/千克之间。该阈值与土壤到蚯蚓的铜生物浓缩系数从1降至0.3相吻合，从而表明铜调节机制明显开始起作用。个体和种群水平的效应仅在土壤铜浓度超过生物标志物阈值时才会出现。因此，能够区分暴露和毒性。所以可以得出结论，中性红测定法在蚯蚓生态毒性研究中具有很大的潜力和相关性。该测定法提供了一个早期预警，可直接与在本实验室研究中观察到的、先于对个体或种群产生不利影响的重要生理变化联系起来。
