开发一种液相色谱/串联质谱法，以分析多种农药暴露导致的丁酰胆碱酯酶抑制作用。
Development of a LC/MS/MS method to analyze butyrylcholinesterase inhibition resulting from multiple pesticide exposure.
作者信息
Sun Jinchun, Lynn Bert C
机构信息
Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 1;877(29):3681-5. doi: 10.1016/j.jchromb.2009.09.009. Epub 2009 Sep 12.
A hybrid LC/MS/MS and proteomics method was developed for the assessment of multiple pesticide exposure. The methodology was based on the analysis of tryptic peptides resulting from inhibited butyrylcholinesterase (BChE) after exposure to pesticides including organophosphates (OPs) and carbamates (CBs). The primary advantage of the assay was its ability to simultaneously examine multiple pesticide exposures in a single analytical experiment. Application of tandem and MS(3) techniques provided identities of the inhibiting pesticide, confirmation and localization of the site of inhibition and relative quantification of phosphorylated peptides present in tryptic digests of equine BChE (eBChE).
开发了一种用于评估多种农药暴露的液相色谱/串联质谱联用（LC/MS/MS）与蛋白质组学方法。该方法基于对接触包括有机磷酸酯（OPs）和氨基甲酸酯（CBs）在内的农药后被抑制的丁酰胆碱酯酶（BChE）产生的胰蛋白酶肽段进行分析。该检测方法的主要优点是能够在单个分析实验中同时检测多种农药暴露情况。串联质谱和MS(3)技术的应用确定了抑制性农药的种类，确认并定位了抑制位点，并对马源丁酰胆碱酯酶（eBChE）胰蛋白酶消化产物中存在的磷酸化肽段进行了相对定量。
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