人体有机磷（OP）暴露的生物标志物。
Biomarkers of organophosphorus (OP) exposures in humans.
机构信息
Department of Medicine, Division Medical Genetics, University of Washington, Seattle, WA 98195-7720, United States.
出版信息
Neurotoxicology. 2011 Oct;32(5):656-60. doi: 10.1016/j.neuro.2011.06.005. Epub 2011 Jul 8.
Abstract
There are ongoing events where aircraft engine lubricant containing tricresyl phosphates (TCPs) contaminates aircraft cabins. Some individuals have experienced tremors or other neurological symptoms that may last for many months following exposures. Mass spectrometric (MS) protocols are being developed to determine the percentage of "biomarker proteins" that are modified by such exposures, specifically on active site serines. Both plasma butyrylcholinesterase (BChE) and red cell acylpeptide hydrolase (APH) are readily inhibited by 2-(ortho-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (CBDP) or phenyl saligenin cyclic phosphate (PSP) and have the potential to provide information about the level of exposure of an individual. We have developed immunomagnetic bead-based single-step purification protocols for both BChE and APH and have characterized the active site serine adducts of BChE by MS.
摘要
正在发生飞机发动机润滑油含三氯联苯 (TCP) 污染飞机客舱的事件。一些人在接触后经历了震颤或其他可能持续数月的神经症状。正在开发质谱 (MS) 方案来确定此类暴露（特别是在活性位点丝氨酸上）修饰的“生物标志物蛋白”的百分比。血浆丁酰胆碱酯酶 (BChE) 和红细胞酰肽水解酶 (APH) 都容易被 2-(邻甲酚基)-4H-1,3,2-苯并二氧磷杂环戊二酮 (CBDP) 或苯亚甲基水杨基磷酸酯 (PSP) 抑制，并且有可能提供关于个体暴露水平的信息。我们已经为 BChE 和 APH 开发了基于免疫磁珠的单步纯化方案，并通过 MS 对 BChE 的活性位点丝氨酸加合物进行了表征。
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