Gan Li-qiang, Fu Yue-xian, Liu Xing, Qiu Lin, Wu Sheng-de, Tian Xiao-fei, Liu Yan, Wei Guang-hui
Department of Pediatric Surgery, Chongqing Children's Hospital, Chongqing Medical University, Chongqing, China.
Toxicol Ind Health. 2009 Aug;25(7):473-8. doi: 10.1177/0748233709345940.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-beta3 (TGF-beta3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-beta3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 microg/kg/day 64 microg/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-beta3 was investigated. We found that the expression of TGF-beta3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-beta3 is related to its pathogenesis.
2,3,7,8-四氯二苯并对二恶英（TCDD）已被证明可诱发腭裂，而该缺陷的分子病因目前仍不清楚。最近，转化生长因子β3（TGF-β3）已被证实对腭突发育起着至关重要的作用。在这项发育毒性研究中，我们研究了2,3,7,8-四氯二苯并对二恶英（TCDD）对胎鼠TGF-β3表达的影响。在胚胎第10天（ED10），将怀孕的C57BL/6小鼠经口暴露于玉米油或TCDD（32微克/千克/天、64微克/千克/天），可诱导腭突发育严重受抑制。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法，研究了TGF-β3的表达情况。我们发现，在TCDD处理组中，TGF-β3的表达逐渐上调。这些结果表明，TCDD暴露可诱发腭裂，TGF-β3的改变与其发病机制有关。
