The Institute of Enology and Viticulture, Yamanashi University, 1-13-1, Kitashin, Kofu, Yamanashi 400-0005, Japan.
Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd, Gui-Shan Township, Taoyuan County 333, Taiwan, ROC.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1914-1917. doi: 10.1099/ijs.0.016873-0. Epub 2009 Sep 25.
Twenty-one homofermentative lactic acid bacteria were isolated from fermented cummingcordia (pobuzihi), a traditional food in Taiwan. The isolates had identical 16S rRNA gene sequences that were distinct from those of other lactobacilli, and their closest neighbours in the 16S rRNA gene sequence phylogenetic tree were strains of Lactobacillus acidipiscis. Levels of DNA-DNA relatedness between representative pobuzihi isolates and strains of L. acidipiscis were 17% and below. Furthermore, the new isolates could be differentiated clearly from L. acidipiscis NBRC 102163T and NBRC 102164 in terms of acid production from L-arabinose, rhamnose, mannitol, lactose and 5-ketogluconate. It was concluded that the new isolates represent a single novel species of the genus Lactobacillus, for which the name Lactobacillus pobuzihii sp. nov. is proposed. The type strain is E100301T (=RIFY 6501T =NBRC 103219T =KCTC 13174T).
从台湾传统发酵食品 cumingcordia(破布子)中分离出 21 株同型发酵乳酸细菌。这些分离株的 16S rRNA 基因序列完全相同,与其他乳杆菌明显不同,在 16S rRNA 基因序列系统发育树中与其最接近的是嗜酸乳杆菌菌株。代表性 pobuzihi 分离株与嗜酸乳杆菌 NBRC 102163T 和 NBRC 102164 之间的 DNA-DNA 同源性水平为 17%及以下。此外,新分离株在产酸方面可明显区分于嗜酸乳杆菌 NBRC 102163T 和 NBRC 102164,可从 L-阿拉伯糖、鼠李糖、甘露醇、乳糖和 5-酮葡萄糖酸产酸。因此,该新分离株代表了乳杆菌属中的一个新种,建议命名为 Lactobacillus pobuzihii sp. nov.。其模式菌株为 E100301T(=RIFY 6501T =NBRC 103219T =KCTC 13174T)。