Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China.
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China.
Molecules. 2022 Mar 14;27(6):1867. doi: 10.3390/molecules27061867.
Ligilactobacillus equi is common in the horse intestine, alleviates the infection of Salmonella, and regulates intestinal flora. Despite this, there have been no genomic studies on this species. Here, we provide the genomic basis for adaptation to the intestinal habitat of this species. We sequenced the genome of L. equi IMAU81196, compared this with published genome information from three strains in NCBI, and analyzed genome characteristics, phylogenetic relationships, and functional genes. The mean genome size of L. equi strains was 2.08 ± 0.09 Mbp, and the mean GC content was 39.17% ± 0.19%. The genome size of L. equi IMAU81196 was 1.95 Mbp, and the GC content was 39.48%. The phylogenetic tree for L. equi based on 1454 core genes showed that the independent branch of strain IMAU81196 was far from the other three strains. In terms of genomic characteristics, single-nucleotide polymorphism (SNP) sites, rapid annotation using subsystem technology (RAST), carbohydrate activity enzymes (CAZy), and predictions of prophage, we showed that strain L. equi JCM 10991T and strain DSM 15833T are not equivalent strains.It is worth mentioning thatthestrain of L. equi has numerous enzymes related to cellulose degradation, and each L. equi strain investigated contained at least one protophage. We speculate that this is the reason why these strains are adapted to the intestinal environment of horses. These results provide new research directions for the future.
马鲛鱼溶藻弧菌是一种常见的肠道菌，能够缓解沙门氏菌感染，调节肠道菌群。尽管如此，目前还没有针对该物种的基因组研究。在这里，我们提供了该物种适应肠道生境的基因组基础。我们对 L. equi IMAU81196 进行了基因组测序，与 NCBI 中三个菌株的已发表基因组信息进行了比较，并分析了基因组特征、系统发育关系和功能基因。L. equi 菌株的平均基因组大小为 2.08 ± 0.09 Mbp，平均 GC 含量为 39.17% ± 0.19%。L. equi IMAU81196 的基因组大小为 1.95 Mbp，GC 含量为 39.48%。基于 1454 个核心基因的 L. equi 系统发育树显示，菌株 IMAU81196 的独立分支远离其他三个菌株。在基因组特征方面，单核苷酸多态性 (SNP) 位点、使用子系统技术快速注释 (RAST)、碳水化合物活性酶 (CAZy) 和噬菌体预测方面，我们表明 L. equi JCM 10991T 和 DSM 15833T 菌株并非等效菌株。值得一提的是，L. equi 菌株具有大量与纤维素降解相关的酶，并且每个调查的 L. equi 菌株至少含有一个原噬菌体。我们推测这就是这些菌株适应马肠道环境的原因。这些结果为未来的研究提供了新的方向。
