Saitoh Y, Yoshimura Y, Nakano K, Miwa N
Cell-Death Control BioTechnology Laboratory, Faculty of Life and environmental Sciences, Prefectural, University of Hiroshima, Shobara, Hiroshima, Japan.
Exp Oncol. 2009 Sep;31(3):156-62.
Hydrogen-dissolved water (HD-water) or platinum nanocolloid (Pt-nc) has been individually expected as a new therapeutic agent for oxidative stress-related diseases, whereas little is known about their combined effects on cancer, which were elucidated in the present study.
HD-water was prepared by microporous gas bubbling, and supplemented with Pt-nc consisting of 0.003-1 ppm Pt and PVP polymers. Antioxidant activities were examined by 1, 1-diphenyl-picrylhydrazyl (DPPH)-radicalscavenging assay. Cytotoxic activities were examined by culturing of tumor and normal cell lines, respectively.
HD-water accelerated the Pt-nc-based DPPH-radical scavenging. Pt-nc-supplemented HD-water inhibited either colony formation efficiencies or colony sizes of human tongue carcinoma cells HSC-4, in contrast to no effects of HD-water alone, Pt-nc alone or Pt-absent PVP, but not appreciably inhibit normal human tongue epithelial-like cells DOK. Pt-nc-supplemented HD-water also suppressed cell population growth of HSC-4 cells of near-confluence (at higher cell densities) in view of decreases in either cell numbers or mitochondrial function, although less markedly than colony formation starting from a sparse-cell state (at lower cell densities). Dissolved hydrogen, oxygen concentration or oxido-reduced potentials of HD-water was decreased, rather decreased or increased by Pt-nc addition, respectively.
Anti-cancer activity of Pt-nc-supplemented HD-water was shown by its preferential cell-growth inhibition to human tongue carcinoma cells HSC-4 over normal human tongue cells DOK, and might be partly attributed to HD-water-caused enhancement of Pt-nc-relevant antioxidant ability. Pt-nc-supplemented HD-water is expected as a novel agent against human tongue cancers due to its cancer progression-repressive abilities.
氢溶解水（HD-水）或铂纳米胶体（Pt-nc）已分别被视为氧化应激相关疾病的新型治疗剂，然而它们对癌症的联合作用却知之甚少，本研究对此进行了阐明。
通过微孔气体鼓泡制备HD-水，并添加由0.003 - 1 ppm铂和聚乙烯吡咯烷酮（PVP）聚合物组成的Pt-nc。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除试验检测抗氧化活性。分别通过培养肿瘤细胞系和正常细胞系检测细胞毒性活性。
HD-水加速了基于Pt-nc的DPPH自由基清除。与单独的HD-水、单独的Pt-nc或不含铂的PVP均无作用相反，添加Pt-nc的HD-水抑制了人舌癌细胞HSC-4的集落形成效率或集落大小，但对正常人舌上皮样细胞DOK没有明显抑制作用。添加Pt-nc的HD-水还抑制了接近汇合状态（较高细胞密度）的HSC-4细胞群体生长，这表现为细胞数量或线粒体功能的降低，尽管其抑制作用不如从稀疏细胞状态（较低细胞密度）开始的集落形成明显。添加Pt-nc后，HD-水中溶解氢、氧浓度或氧化还原电位分别降低、降低或升高。
添加Pt-nc的HD-水对人舌癌细胞HSC-4的细胞生长抑制作用优于正常人舌细胞DOK，显示出抗癌活性，这可能部分归因于HD-水导致的与Pt-nc相关的抗氧化能力增强。由于其具有抑制癌症进展的能力，添加Pt-nc的HD-水有望成为一种新型的抗人舌癌药物。
