Osaka Buturyo College, Nishi-ku, Sakai 593-8328, Japan.
Oncol Rep. 2010 Dec;24(6):1463-70. doi: 10.3892/or_00001006.
In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidation-reduction potential -650 mV, normal water: +100-200 mV) using a microporous-filter hydrogen-jetting device. We showed that hydrogen water erased ROS indispensable for tumor cell growth by ESR/spin trap, the redox indicator CDCFH-DA assay, and was cytotoxic to Ehrlich ascites tumor cells as assessed by WST-8 assay, crystal violet dye stain and scanning electron microscopy, after 24-h or 48-h incubation sequent to warming at 37°C or 42°C. Hydrogen water supplemented with platinum colloid (0.3 ppm Pt in 4% polyvinylpyrrolidone) had more antitumor activity than hydrogen water alone, mineral water alone (15.6%), hydrogen water plus mineral water, or platinum colloid alone as observed by decreased cell numbers, cell shrinkage and pycnosis (nuclear condensation)/karyorrhexis (nuclear fragmentation) indicative of apoptosis, together with cell deformation and disappearance of microvilli on the membrane surface. These antitumor effects were promoted by combination with hyperthermia at 42°C. Thus, the nano-bubble hydrogen water with platinum colloid is potent as an anti-tumor agent.
为了通过减少氢气的活性来消除与肿瘤细胞增殖有关的活性氧（ROS），我们使用微孔过滤器氢气喷射装置开发了一种含有纳米气泡（直径：<900nm 占 71%/群体）的功能性水，其氢气浓度为 1.1-1.5ppm（理论最大值：1.6ppm），具有还原能力（氧化还原电位-650mV，正常水：+100-200mV）。我们通过 ESR/自旋陷阱、氧化还原指示剂 CDCFH-DA 测定法以及 WST-8 测定法、结晶紫染料染色和扫描电子显微镜观察，显示氢气水可以消除肿瘤细胞生长所必需的 ROS，经过 24 小时或 48 小时孵育后，在 37°C 或 42°C 下升温。与单独的氢气水、矿泉水（15.6%）、氢气水加矿泉水或单独的胶体铂相比，补充有胶体铂（4%聚乙烯吡咯烷酮中的 0.3ppmPt）的氢气水具有更强的抗肿瘤活性，观察到细胞数量减少、细胞收缩和固缩（核浓缩）/核碎裂（核碎片），表明细胞凋亡，同时细胞膜表面的细胞变形和微绒毛消失。这些抗肿瘤作用通过与 42°C 的热疗联合得到促进。因此，含胶体铂的纳米气泡氢气水是一种有效的抗肿瘤剂。
