Kuksis Arnis, Suomela Jukka-Pekka, Tarvainen Marko, Kallio Heikki
Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2009;580:39-91. doi: 10.1007/978-1-60761-325-1_4.
Various analytical techniques have been adopted for the isolation and identification of the oxolipids and for determining their functionality. Gas chromatography in combination with mass spectrometry (MS) has been specifically utilized in analysis of isoprostanes and other low molecular weight oxolipids, although it requires derivatization of the solutes. In contrast, liquid chromatography (LC) in combination with on-line MS has proven to be well suited for analysis of intact oxolipids without (or minimal) derivatization. LC-MS has also been helpful for the identification of lipidomic changes resulting from covalent binding of lipid ester core aldehydes to amino lipids, amino acids, peptides, and proteins. This chapter reviews the use of the above techniques for lipidomic analysis of the autoxidation products of cholesteryl esters and glycerolipids as practiced in the authors' laboratories.
已经采用了各种分析技术来分离和鉴定氧化脂质，并确定它们的功能。气相色谱与质谱联用（MS）已专门用于分析异前列腺素和其他低分子量氧化脂质，尽管这需要对溶质进行衍生化。相比之下，液相色谱（LC）与在线MS联用已被证明非常适合分析完整的氧化脂质，无需（或只需极少）衍生化。LC-MS也有助于鉴定由于脂质酯核心醛与氨基脂质、氨基酸、肽和蛋白质的共价结合而导致的脂质组学变化。本章回顾了作者实验室中使用上述技术对胆固醇酯和甘油脂质的自氧化产物进行脂质组学分析的情况。
