新流式细胞分析检测到手术和滑膜切除术来源的类风湿滑膜巨噬细胞上 TNF 受体的不同表达水平。
Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.
机构信息
First Department of Internal Medicine, Nagasaki University Hospital, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan,
出版信息
Cytotechnology. 2009 Jul;60(1-3):161-4. doi: 10.1007/s10616-009-9229-5. Epub 2009 Sep 26.
Abstract
TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TNF receptors on the heterogeneous rheumatoid synovial cells. Using this system, we detected a higher population of macrophages and a greater TNF receptor expression on the synovial macrophages derived from a synovectomy in comparison to the findings obtained from knee joint replacement surgery.
摘要
TNFalpha 在类风湿关节炎的发病机制中起着至关重要的作用。检查 TNFalpha 的配体 TNF 受体的表达非常重要。在这项研究中，我们开发了一种使用 CD45 和 CD14 单克隆抗体的三色流式细胞分析，以简单地检测异质性类风湿滑膜细胞上 TNF 受体的表达。使用该系统，我们在滑膜切除术中检测到更高比例的巨噬细胞和滑膜巨噬细胞上的 TNF 受体表达，与膝关节置换术中的发现相比。
相似文献
1
Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.新流式细胞分析检测到手术和滑膜切除术来源的类风湿滑膜巨噬细胞上 TNF 受体的不同表达水平。
Cytotechnology. 2009 Jul;60(1-3):161-4. doi: 10.1007/s10616-009-9229-5. Epub 2009 Sep 26.
2
Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis.类风湿关节炎中CD16⁺血液单核细胞和滑膜组织巨噬细胞上Toll样受体2的表达
Arthritis Rheum. 2004 May;50(5):1457-67. doi: 10.1002/art.20219.
3
Expression and regulation of macrophage-inducible C-type lectin in human synovial macrophages.巨噬细胞诱导型C型凝集素在人滑膜巨噬细胞中的表达与调控
Cent Eur J Immunol. 2020;45(4):377-381. doi: 10.5114/ceji.2020.103411. Epub 2021 Jan 30.
4
Rheumatoid arthritis synovial fluid macrophages express decreased tumor necrosis factor-related apoptosis-inducing ligand R2 and increased decoy receptor tumor necrosis factor-related apoptosis-inducing ligand R3.类风湿性关节炎滑液巨噬细胞表达的肿瘤坏死因子相关凋亡诱导配体R2减少，而诱饵受体肿瘤坏死因子相关凋亡诱导配体R3增加。
Arthritis Rheum. 2003 Nov;48(11):3096-101. doi: 10.1002/art.11302.
5
Mast cell activation and its relation to proinflammatory cytokine production in the rheumatoid lesion.肥大细胞活化及其与类风湿性病变中促炎细胞因子产生的关系。
Arthritis Res. 2000;2(1):65-74. doi: 10.1186/ar70.
6
Abundant expression of common cytokine receptor gamma chain (CD132) in rheumatoid joints.类风湿关节中共同细胞因子受体γ链（CD132）的大量表达。
J Rheumatol. 2001 Feb;28(2):240-4.
7
Tumour necrosis factor alpha (TNF-alpha) interferes with Fas-mediated apoptotic cell death on rheumatoid arthritis (RA) synovial cells: a possible mechanism of rheumatoid synovial hyperplasia and a clinical benefit of anti-TNF-alpha therapy for RA.肿瘤坏死因子α（TNF-α）干扰类风湿关节炎（RA）滑膜细胞上Fas介导的凋亡性细胞死亡：类风湿滑膜增生的一种可能机制及抗TNF-α治疗对RA的临床益处。
Cytokine. 2000 Mar;12(3):281-8. doi: 10.1006/cyto.1999.0552.
8
Enhanced expression of tumor necrosis factor receptor mRNA and protein in mononuclear cells isolated from rheumatoid arthritis synovial joints.类风湿性关节炎滑膜关节分离出的单核细胞中肿瘤坏死因子受体mRNA和蛋白的表达增强。
Eur J Immunol. 1992 Jul;22(7):1907-12. doi: 10.1002/eji.1830220734.
9
Immunolocalization of bradykinin receptors on human synovial tissue.缓激肽受体在人滑膜组织上的免疫定位
Immunopharmacology. 1997 Jun;36(2-3):121-5. doi: 10.1016/s0162-3109(97)00010-6.
10
Site-specific intraoperative efficacy of arthroscopic knee joint synovectomy in rheumatoid arthritis.类风湿关节炎中关节镜下膝关节滑膜切除术的特定部位术中疗效
Arthroscopy. 2005 Oct;21(10):1209-18. doi: 10.1016/j.arthro.2005.06.014.
引用本文的文献
1
Crosstalk between FLS and chondrocytes is regulated by HIF-2α-mediated cytokines in arthritis.在关节炎中，滑膜成纤维细胞（FLS）与软骨细胞之间的相互作用由低氧诱导因子-2α（HIF-2α）介导的细胞因子调控。
Exp Mol Med. 2015 Dec 4;47(12):e197. doi: 10.1038/emm.2015.88.
2
Differential pro-inflammatory responses of TNF-α receptors (TNFR1 and TNFR2) on LOX-1 signalling.肿瘤坏死因子-α受体（TNFR1和TNFR2）对凝集素样氧化低密度脂蛋白受体-1（LOX-1）信号传导的不同促炎反应
Mol Biol Rep. 2015 Jun;42(6):1039-47. doi: 10.1007/s11033-014-3841-y. Epub 2014 Nov 23.
本文引用的文献
1
Review: Immune cells and mediators of inflammatory arthritis.综述：炎性关节炎的免疫细胞与介质
Autoimmunity. 2008 Apr;41(3):224-9. doi: 10.1080/08916930701694717.
2
Cells of the synovium in rheumatoid arthritis. Synovial fibroblasts.类风湿关节炎中的滑膜细胞。滑膜成纤维细胞。
Arthritis Res Ther. 2007;9(6):223. doi: 10.1186/ar2337.
3
Molecular and cellular basis of rheumatoid joint destruction.类风湿性关节破坏的分子和细胞基础。
Immunol Lett. 2006 Jul 15;106(1):8-13. doi: 10.1016/j.imlet.2006.04.011. Epub 2006 May 24.
4
Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-gamma through JAK/STAT pathway by translational regulation.干扰素-γ通过JAK/STAT途径经翻译调控对TRAIL介导的成纤维样滑膜细胞凋亡产生显著抑制作用。
J Lab Clin Med. 2006 Apr;147(4):182-90. doi: 10.1016/j.lab.2005.12.001.
5
Synovial fibroblasts: key players in rheumatoid arthritis.滑膜成纤维细胞：类风湿关节炎的关键参与者。
Rheumatology (Oxford). 2006 Jun;45(6):669-75. doi: 10.1093/rheumatology/kel065. Epub 2006 Mar 27.
6
Suppressive effect of leflunomide metabolite (A77 1726) on metalloproteinase production in IL-1beta stimulated rheumatoid synovial fibroblasts.来氟米特代谢物（A77 1726）对白细胞介素-1β刺激的类风湿性滑膜成纤维细胞中金属蛋白酶产生的抑制作用。
Clin Exp Immunol. 2004 Sep;137(3):612-6. doi: 10.1111/j.1365-2249.2004.02555.x.
7
Osteoprotegerin (OPG) acts as an endogenous decoy receptor in tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of fibroblast-like synovial cells.骨保护素（OPG）在肿瘤坏死因子相关凋亡诱导配体（TRAIL）介导的成纤维细胞样滑膜细胞凋亡中作为内源性诱饵受体发挥作用。
Clin Exp Immunol. 2004 Aug;137(2):430-6. doi: 10.1111/j.1365-2249.2004.02534.x.
8
Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid synovial fibroblasts.白细胞介素-18可诱导类风湿性滑膜成纤维细胞产生血清淀粉样蛋白A（SAA）。
Life Sci. 2004 Feb 13;74(13):1671-9. doi: 10.1016/j.lfs.2003.08.025.
9
Cytokines regulate fibroblast-like synovial cell differentiation to adipocyte-like cells.细胞因子调节成纤维样滑膜细胞向脂肪样细胞的分化。
Rheumatology (Oxford). 2004 Apr;43(4):448-52. doi: 10.1093/rheumatology/keh092. Epub 2004 Jan 20.
10
Rheumatoid arthritis: regulation of synovial inflammation.类风湿性关节炎：滑膜炎症的调节
Int J Biochem Cell Biol. 2004 Mar;36(3):372-8. doi: 10.1016/s1357-2725(03)00259-0.
Zotero 插件