新流式细胞分析检测到手术和滑膜切除术来源的类风湿滑膜巨噬细胞上 TNF 受体的不同表达水平。
Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.
机构信息
First Department of Internal Medicine, Nagasaki University Hospital, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan,
出版信息
Cytotechnology. 2009 Jul;60(1-3):161-4. doi: 10.1007/s10616-009-9229-5. Epub 2009 Sep 26.
Abstract
TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TNF receptors on the heterogeneous rheumatoid synovial cells. Using this system, we detected a higher population of macrophages and a greater TNF receptor expression on the synovial macrophages derived from a synovectomy in comparison to the findings obtained from knee joint replacement surgery.
摘要
TNFalpha 在类风湿关节炎的发病机制中起着至关重要的作用。检查 TNFalpha 的配体 TNF 受体的表达非常重要。在这项研究中，我们开发了一种使用 CD45 和 CD14 单克隆抗体的三色流式细胞分析，以简单地检测异质性类风湿滑膜细胞上 TNF 受体的表达。使用该系统，我们在滑膜切除术中检测到更高比例的巨噬细胞和滑膜巨噬细胞上的 TNF 受体表达，与膝关节置换术中的发现相比。
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本文引用的文献
1
Review: Immune cells and mediators of inflammatory arthritis.
Autoimmunity. 2008 Apr;41(3):224-9. doi: 10.1080/08916930701694717.
2
Cells of the synovium in rheumatoid arthritis. Synovial fibroblasts.
Arthritis Res Ther. 2007;9(6):223. doi: 10.1186/ar2337.
3
Molecular and cellular basis of rheumatoid joint destruction.
Immunol Lett. 2006 Jul 15;106(1):8-13. doi: 10.1016/j.imlet.2006.04.011. Epub 2006 May 24.
4
Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-gamma through JAK/STAT pathway by translational regulation.
J Lab Clin Med. 2006 Apr;147(4):182-90. doi: 10.1016/j.lab.2005.12.001.
5
Synovial fibroblasts: key players in rheumatoid arthritis.
Rheumatology (Oxford). 2006 Jun;45(6):669-75. doi: 10.1093/rheumatology/kel065. Epub 2006 Mar 27.
6
Suppressive effect of leflunomide metabolite (A77 1726) on metalloproteinase production in IL-1beta stimulated rheumatoid synovial fibroblasts.
Clin Exp Immunol. 2004 Sep;137(3):612-6. doi: 10.1111/j.1365-2249.2004.02555.x.
7
Osteoprotegerin (OPG) acts as an endogenous decoy receptor in tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of fibroblast-like synovial cells.
Clin Exp Immunol. 2004 Aug;137(2):430-6. doi: 10.1111/j.1365-2249.2004.02534.x.
8
Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid synovial fibroblasts.
Life Sci. 2004 Feb 13;74(13):1671-9. doi: 10.1016/j.lfs.2003.08.025.
9
Cytokines regulate fibroblast-like synovial cell differentiation to adipocyte-like cells.
Rheumatology (Oxford). 2004 Apr;43(4):448-52. doi: 10.1093/rheumatology/keh092. Epub 2004 Jan 20.
10
Rheumatoid arthritis: regulation of synovial inflammation.
Int J Biochem Cell Biol. 2004 Mar;36(3):372-8. doi: 10.1016/s1357-2725(03)00259-0.
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