Zic 家族成员 2 的表达状态作为口腔鳞状细胞癌的预后标志物。
Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma.
机构信息
Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.
出版信息
J Cancer Res Clin Oncol. 2010 Apr;136(4):553-9. doi: 10.1007/s00432-009-0689-y. Epub 2009 Sep 27.
PURPOSE
To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC).
METHODS
ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status.
RESULTS
Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases.
CONCLUSIONS
These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.
目的
确定 ZIC2 在口腔鳞状细胞癌（OSCC）中的作用。
方法
采用实时定量逆转录聚合酶链反应、Western blot 和免疫组织化学（IHC）分析 74 例原发性 OSCC、20 例口腔癌前病变（OPL）和 5 种 OSCC 衍生细胞系（HSC-2、HSC-3、OK-92、H1 和 Sa3）中 ZIC2 mRNA 和蛋白的表达情况。此外，我们还评估了 ZIC2 在 OSCC 中的 IHC 评分与临床病理状态之间的相关性。
结果
与正常对照组相比，OSCC 衍生细胞系（P < 0.05）、原发性 OSCC（P < 0.05）和 OPL（P < 0.05）中 ZIC2 的表达明显上调。在分析的临床变量中，ZIC2 表达与 OSCC 的组织病理学类型有关。此外，ZIC2 阳性病例和 ZIC2 阴性病例的生存率差异有统计学意义。
结论
这些结果表明，ZIC2 的表达与 OSCC 的分化类型以及诊断有关，可能是 OSCC 的一个潜在预后指标和治疗靶点。
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