Prado Susana, Montes Jaime, Romalde Jesús L, Barja Juan L
Department of Microbiology and Parasitology, Institute of Aquaculture, University of Santiago de Compostela, Spain.
Int Microbiol. 2009 Jun;12(2):107-14.
A total of 523 bacterial strains were isolated during a 4-year period from mollusc hatcheries (flat oyster and clams) in Galicia (NW Spain). All of the strains were tested for their antibacterial activity against three larval pathogens (Vibrio anguillarum USC-72, V. neptunius PP-145.98, and Vibrio sp. PP-203). Of the isolates, 52 inhibited at least one of the target strains, and 11 inhibited all of them. The main source of active strains was oyster larvae, followed by water, tank surfaces, spat, and broodstock. Four similar strains, belonging to the genus Phaeobacter, showed the strongest activity. Strain PP-154, selected as representative of this group, displayed a wide spectrum of inhibitory activity against aquaculture pathogens, especially against members of the genus Vibrio, which is responsible for the most larval deaths. The inhibitory ability of such strain on solid medium was confirmed in seawater experiments, and the optimal conditions for antibacterial activity were established. These strains are promising probiotics for aquaculture facilities. Their potential benefit is based on the capacity to control the proliferation of a variety of aquaculture bacterial pathogens in mollusc larval cultures.
在4年时间里，从西班牙西北部加利西亚的软体动物孵化场（扁牡蛎和蛤）分离出了总共523株细菌菌株。对所有菌株针对三种幼虫病原体（鳗弧菌USC - 72、海神弧菌PP - 145.98和弧菌属PP - 203）进行了抗菌活性测试。在分离出的菌株中，52株至少抑制了一种目标菌株，11株抑制了所有目标菌株。活性菌株的主要来源是牡蛎幼虫，其次是水、养殖池表面、贝苗和亲鱼。四种属于海杆菌属的相似菌株表现出最强的活性。选为该组代表的菌株PP - 154对水产养殖病原体表现出广泛的抑制活性，尤其是对弧菌属成员，该属是导致大多数幼虫死亡的原因。在海水实验中证实了该菌株在固体培养基上的抑制能力，并确定了抗菌活性的最佳条件。这些菌株是水产养殖设施中有前景的益生菌。它们的潜在益处基于控制软体动物幼虫养殖中多种水产养殖细菌病原体增殖的能力。
