Department of Anatomy, Histology and Embryology, Medical School, University of Ioannina, Ioannina 451 10, Greece.
Microsc Res Tech. 2010 May;73(5):495-502. doi: 10.1002/jemt.20788.
The effects of the administration of parenteral glucose on the postnatal glycogen autophagic activity and its relationship to the general autophagic activity, were studied in newborn rat liver using electron microscopy and biochemical methods. Glucose abolished the normal postnatal hypoglycemia and preserved the hepatocytic hyaloplasmic glycogen to the levels of birth. It also inhibited the normal postnatal increase in the number and volume of autophagic vacuoles. Glucose especially decreased the rate of postnatal development of the glycogen-containing autophagic vacuoles. This decrease was greater than that of the autophagic vacuoles in general. In the control animals at the age of 6 h, the total volume of the glycogen-containing autophagic vacuoles accounted for 87% of the autophagic vacuoles in general, whereas in the glucose-treated animals of the same age, for only 62%. The results of this and previous studies support the view that the general autophagic activity that develops in the immediate postnatal period in rat hepatocytes is mainly expressed as glycogen autophagic activity selectively inhibited by glucose.
用电子显微镜和生化方法研究了新生大鼠肝组织中葡萄糖的给予对糖原自噬活性及其与一般自噬活性的关系。葡萄糖消除了正常的新生儿低血糖，并将肝细胞透明质糖原维持在出生时的水平。它还抑制了正常的新生儿自噬小体数量和体积的增加。葡萄糖特别降低了含糖原的自噬小体的产后发育速度。这种减少大于一般自噬小体的减少。在对照组动物出生后 6 小时，含糖原的自噬小体的总体积占一般自噬小体的 87%，而在相同年龄的葡萄糖处理动物中，仅占 62%。本研究和以前的研究结果支持这样一种观点，即在新生大鼠肝细胞中立即发育的一般自噬活性主要表现为葡萄糖选择性抑制的糖原自噬活性。
