Department of Food Science, North Carolina State University, Raleigh, North Carolina 27695, USA.
Foodborne Pathog Dis. 2010 Jan;7(1):97-101. doi: 10.1089/fpd.2009.0362.
Vibrio vulnificus strains (n = 469) isolated from the Gulf of Mexico oysters and waters over a period of 2 years were subjected to phenotypic and genotypic characterizations. Of the strains that could be definitively genotyped (n = 465), 58% were classified as genotype A, 29% as genotype B, and 13% as genotype A/B by 16S rRNA genotyping. When the same strain bank was characterized by virulence-correlated gene (vcg) typing, 65% were genotype E while 35% were genotype C. Further analysis focusing on strains falling into typical genotype categories (i.e., 16S rRNA types A or B, excluding type A/B strains) showed a high degree of concordance (93%) when comparing the two genotyping methods. d-Mannitol fermentation was also predictive of genotype, with an 86% agreement between 16S rRNA genotype and mannitol fermentation patterns, and an 85% agreement between vcg genotype and mannitol fermentation patterns. d-Mannitol fermentation should be considered as a simple and less expensive alternative to screen V. vulnificus isolates for virulence potential, particularly when analyzing large strain banks.
在为期两年的时间里，从墨西哥湾牡蛎和水域中分离出的 469 株创伤弧菌菌株进行了表型和基因型特征分析。在可明确进行基因分型的菌株中（n=465），58%被归类为 A 基因型，29%为 B 基因型，13%为 A/B 基因型，这是通过 16S rRNA 基因分型得出的结果。当同一菌株库通过与毒力相关的基因（vcg）分型进行特征分析时，65%为 E 基因型，35%为 C 基因型。进一步的分析集中在属于典型基因型类别的菌株上（即 16S rRNA 类型 A 或 B，不包括 A/B 型菌株），两种基因分型方法的比较显示出高度一致性（93%）。d-甘露醇发酵也可预测基因型，16S rRNA 基因型与甘露醇发酵模式之间的一致性为 86%，vcg 基因型与甘露醇发酵模式之间的一致性为 85%。d-甘露醇发酵应该被认为是一种简单且廉价的替代方法，用于筛选创伤弧菌菌株的毒力潜力，特别是在分析大型菌株库时。
