Escuela Nacional de Ciencias Biológicas, Mexico.
Int J Environ Health Res. 2010 Dec;20(6):395-405. doi: 10.1080/09603123.2010.491856.
The presence of Vibrio vulnificus was analyzed in oyster and estuarine water samples from Mexico by PCR amplification of the vvhA gene and some putative virulence factors were tested. Samples were collected from 12 different sampling points over a one-year period; 31% samples were positive for V. vulnificus and all isolates were identified as biotype 1. All strains were cytotoxic and proteolytic, 98% showed adherence to epithelial cells, 91.4% were DNase-positive, 77.6% were mucinase-positive, 97.8% were lecithinase-positive and 79.8% were lipase positive. Regarding colony morphology, 51% strains were opaque, 20% were translucid, 28% were both opaque and translucid, and 80.8% showed a capsule. This is the first report on the isolation of V. vulnificus strains from environmental samples in Mexico, which may pose a health risk for local fisherman and seafood consumers.
采用 PCR 扩增 vvhA 基因的方法，对来自墨西哥牡蛎和河口水中的创伤弧菌进行了分析，并对一些可能的毒力因子进行了检测。在一年的时间里，从 12 个不同的采样点采集了样本；31%的样本中检测到创伤弧菌，所有分离株均被鉴定为生物型 1。所有菌株均具有细胞毒性和蛋白水解活性，98%的菌株表现出对上皮细胞的黏附性，91.4%的菌株呈 DNA 酶阳性，77.6%的菌株呈粘蛋白酶阳性，97.8%的菌株呈卵磷脂酶阳性，79.8%的菌株呈脂肪酶阳性。关于菌落形态，51%的菌株呈不透明状，20%的菌株呈半透明状，28%的菌株既不透明也半透明，80.8%的菌株具有荚膜。这是墨西哥首次从环境样本中分离出创伤弧菌菌株的报告，这可能对当地渔民和海鲜消费者的健康构成威胁。
