一种与2A型多发性内分泌腺瘤病（MEN2A）基因座紧密连锁的新型DNA标记（D10S94）。
A new DNA marker (D10S94) very tightly linked to the multiple endocrine neoplasia type 2A (MEN2A) locus.
作者信息
Goodfellow P J, Myers S, Anderson L L, Brooks-Wilson A R, Simpson N E
机构信息
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
出版信息
Am J Hum Genet. 1990 Dec;47(6):952-6.
Combined somatic cell hybrid and linkage studies between D10S94 and five pericentromeric loci (FNRB, D10Z1, MEN2A, RBP3, and D10S15) have localized the new DNA sequence pcl1/A1S-6-c23 at D10S94 to 10q11.2. No recombinants were observed between D10S94 and D10Z1 or MEN2A. D10S94 maps in proximal 10q11.2 very near to MEN2A. There are three possible orders for the six loci that we investigated from the centromeric region of chromosome 10. At present the genetic data do not allow us to order MEN2A with respect to D10Z1 and D10S94. The three possible orders are FNRB-D10Z1-D10S94-MEN2A-RBP3-D10S15, FNRB-D10Z1-MEN2A-D10S94-RBP3-D10S15, and FNRB-MEN2A-D10Z1-D10S94-RBP3-D10S15. In view of the fact that no recombinants between D10S94 and MEN2A or between D10S94 and D10Z1 were observed, the combined haplotypes formed from RFLPs and D10Z1 and D10S94 will increase the informativeness and accuracy of genotype prediction for at-risk members of the families having the MEN 2A syndrome, particularly when the affected parent is female. The localization of D10S94 with respect to MEN2A will prove valuable in experiments directed toward cloning the MEN2A locus.
体细胞杂交与连锁研究相结合，研究了D10S94与五个着丝粒周围位点（FNRB、D10Z1、MEN2A、RBP3和D10S15）之间的关系，已将D10S94处的新DNA序列pcl1/A1S - 6 - c23定位到10q11.2。在D10S94与D10Z1或MEN2A之间未观察到重组。D10S94定位于10q11.2近端，非常靠近MEN2A。我们从10号染色体的着丝粒区域研究的六个位点有三种可能的顺序。目前，遗传数据不允许我们确定MEN2A相对于D10Z1和D10S94的顺序。这三种可能的顺序是FNRB - D10Z1 - D10S94 - MEN2A - RBP3 - D10S15、FNRB - D10Z1 - MEN2A - D10S94 - RBP3 - D10S15和FNRB - MEN2A - D10Z1 - D10S94 - RBP3 - D10S15。鉴于在D10S94与MEN2A之间或D10S94与D10Z1之间未观察到重组，由限制性片段长度多态性（RFLP）以及D10Z1和D10S94形成的组合单倍型将提高对患有MEN 2A综合征家庭中高危成员基因型预测的信息量和准确性，特别是当患病父母为女性时。D10S94相对于MEN2A的定位在针对克隆MEN2A基因座的实验中将被证明是有价值的。
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