[TX小鼠（一种肝脏疾病动物模型）铜代谢与肝损伤的研究]

[Study of copper metabolism and liver damage in TX Mice-an animal model for liver disease].

作者信息

Chen Xi, Wang Chu-huai, Feng Yan-qing, Tang Qi-qiang, Xie Qiu-you, Liang Qi, Liang Xiu-ling

机构信息

Department of Rehabilitation Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2009 Sep;17(9):688-90.

PMID:19785958

Abstract

OBJECTIVE

To provide right time points in selection of right aged animals and the normal physiological data of TX mice.

METHODS

7-12 months old TX and DL mice were studied, each group contained 3 female and 3 male mice of TX or DL mice. The concentration of copper in the serum, dry tissues (liver, brain and kidney), together with copper biochemistry indexes were measured. The liver histopathology was observed under light microscopy and electron microscope.

RESULTS

Transaminase increased significantly only in 10 and 11-month- old (AST(TX10) = 218.3 U/L, AST(TX11) = 197.5 U/L, AST(DL10) = 171.5 U/L, AST(DL11) = 165.0 U/L, P(10) less than 0.001, P(11) = 0.022), but the copper concentration of liver, brain and kidney was significantly increased during 7-12 month old (the average concentration of copper, Liver(TX) = (750.0 +/- 85.5) mg/kg, Brain(TX) = (39.7 +/- 2.2)mg/kg, Kidney(TX) = (29.8 +/- 5.0) mg/kg, Liver(DL) = (11.6 +/- 1.5) mg/kg, Brain(DL) = (16.8 +/- 0.9) mg/kg, Kidney(DL) = (14.2 +/- 1.0) mg/kg, t = 21.16, 23.60, 7.47, for all these organs P less than 0.05).

CONCLUSION

TX mice is a suitable model of liver disease with natural recovery, so selecting animal model of suitable time point is very important.

摘要

目的

为选择合适年龄的动物提供正确的时间点，并获取TX小鼠的正常生理数据。

方法

对7至12月龄的TX和DL小鼠进行研究，每组包含3只雌性和3只雄性TX或DL小鼠。测量血清、干燥组织（肝脏、大脑和肾脏）中的铜浓度以及铜生化指标。在光学显微镜和电子显微镜下观察肝脏组织病理学。

结果

仅在10和11月龄时转氨酶显著升高（AST(TX10)=218.3 U/L，AST(TX11)=197.5 U/L，AST(DL10)=171.5 U/L，AST(DL11)=165.0 U/L，P(10)<0.001，P(11)=0.022），但在7至12月龄期间肝脏、大脑和肾脏中的铜浓度显著升高（铜的平均浓度，肝脏(TX)=(750.0±85.5)mg/kg，大脑(TX)=(39.7±2.2)mg/kg，肾脏(TX)=(29.8±5.0)mg/kg，肝脏(DL)=(11.6±1.5)mg/kg，大脑(DL)=(16.8±0.9)mg/kg，肾脏(DL)=(14.2±1.0)mg/kg，t=21.16、23.60、7.47，所有这些器官P<0.05）。