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N255 是一种关键残基，可被一种单克隆抗体识别，该抗体可预防鼠疫耶尔森氏菌感染。

N255 is a key residue for recognition by a monoclonal antibody which protects against Yersinia pestis infection.

机构信息

Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.

出版信息

Vaccine. 2009 Nov 23;27(50):7073-9. doi: 10.1016/j.vaccine.2009.09.061. Epub 2009 Sep 26.

DOI:10.1016/j.vaccine.2009.09.061
PMID:19786138
Abstract

Mab7.3 to Yersinia pestis LcrV antigen (LcrV(Ype)) protected J774A.1 macrophages in vitro from killing by a Yersinia pseudotuberculosis strain expressing LcrV(Ype). Of 4 site-directed mutations in the coiled-coil region (148-169) and 7 mutations in the 225-255 sequence of LcrV(Ype), only the mutation of N255 to D255, abrogated the binding of Mab7.3 and reduced its protective capacity against plague. Since the Mab7.3 epitope in LcrV(Ype) (135-275) encompasses a region (136-180) thought to be exposed on the injectisome, we suggest that Mab7.3 protects by binding to LcrV(Ype) and interfering with protein-protein interactions necessary for type three secretion.

摘要

Mab7.3 对鼠疫耶尔森菌 LcrV 抗原（LcrV(Ype)）的识别保护了体外的 J774A.1 巨噬细胞免受表达 LcrV(Ype)的鼠疫假结核耶尔森菌的杀伤。在 LcrV(Ype)的卷曲螺旋区（148-169）的 4 个定点突变和 225-255 序列的 7 个突变中，只有 N255 突变为 D255，才会导致 Mab7.3 的结合丧失，并降低其对鼠疫的保护能力。由于 Mab7.3 在 LcrV(Ype)（135-275）上的表位（136-180）被认为暴露在注射器上，我们推测 Mab7.3 通过与 LcrV(Ype)结合并干扰必要的蛋白-蛋白相互作用来保护机体免受 III 型分泌的影响。

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