Division of Avian Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Vaccine. 2009 Nov 23;27(50):7046-52. doi: 10.1016/j.vaccine.2009.09.065. Epub 2009 Sep 26.
A fowlpox virus expressing the chicken infectious bronchitis virus (IBV) S1 gene of the LX4 strain (rFPV-IBVS1) and a fowlpox virus co-expressing the S1 gene and the chicken type II interferon gene (rFPV-IBVS1-ChIFNgamma) were constructed. These viruses were assessed for their immunological efficacy on specific-pathogen-free (SPF) chickens challenged with a virulent IBV. Although the antibody levels in the rFPV-IBVS1-ChIFNgamma-vaccinated group were lower than those in the attenuated live IB vaccine H120 group and the rFPV-IBVS1 group, the rFPV-IBVS1-ChIFNgamma provided the strongest protection against an IBV LX4 virus challenge (15 out of 16 chickens immunized with rFPV-IBVS1-ChIFNgamma were protected), followed by the attenuated live IB vaccine (13/16 protected) and the rFPV-IBVS1 (12/16 protected). Compared to those of the rFPV-IBVS1 and the attenuated live IB vaccine groups, chickens in the rFPV-IBVS1-ChIFNgamma group eliminated virus more quickly and decreased the presence of viral antigen more significantly in renal tissue. Examination of affected tissues revealed abnormalities in the liver, spleen, kidney, lung and trachea of chickens vaccinated with the attenuated live IB vaccine and the rFPV-IBVS1 vaccine. In rFPV-IBVS1-ChIFNgamma-vaccinated chickens, pathological changes were also observed in those organs, but were milder and lasted shorter. The lesions in the mock control group were the most severe and lasted for at least 20 days. This study demonstrated that chicken type II interferon increased the immunoprotective efficacy of rFPV-IBVS1-ChIFNgamma and normal weight gain in vaccinated chickens although it inhibited serum antibody production.
构建了一株表达鸡传染性支气管炎病毒（IBV）LX4 株 S1 基因的禽痘病毒（rFPV-IBVS1）和一株共表达 S1 基因和鸡Ⅱ型干扰素基因（rFPV-IBVS1-ChIFNγ）的禽痘病毒。评估了这些病毒在 SPF 鸡攻毒强毒 IBV 时的免疫效力。虽然 rFPV-IBVS1-ChIFNγ 疫苗组的抗体水平低于弱毒活 IB 疫苗 H120 组和 rFPV-IBVS1 组，但 rFPV-IBVS1-ChIFNγ 对 IBV LX4 病毒攻毒提供了最强的保护（16 只免疫 rFPV-IBVS1-ChIFNγ 的鸡中有 15 只得到保护），其次是弱毒活 IB 疫苗（13/16 只得到保护）和 rFPV-IBVS1（12/16 只得到保护）。与 rFPV-IBVS1 和弱毒活 IB 疫苗组相比，rFPV-IBVS1-ChIFNγ 组的鸡更快地清除病毒，肾脏组织中病毒抗原的存在减少更为明显。受影响组织的检查发现，弱毒活 IB 疫苗和 rFPV-IBVS1 疫苗接种鸡的肝脏、脾脏、肾脏、肺和气管有异常。rFPV-IBVS1-ChIFNγ 疫苗接种鸡的这些器官也观察到病理变化，但较轻且持续时间较短。在模拟对照组中，病变最严重，至少持续 20 天。本研究表明，鸡Ⅱ型干扰素增强了 rFPV-IBVS1-ChIFNγ 的免疫保护效力，尽管它抑制了血清抗体的产生，但增加了接种鸡的正常体重增加。
