一项基于新型 HIV 辅助性 T 细胞表位的疫苗在一项针对未感染 HIV 的成年人的 I 期临床试验中引发了细胞因子分泌的 HIV 特异性 CD4+ T 细胞。
A novel HIV T helper epitope-based vaccine elicits cytokine-secreting HIV-specific CD4+ T cells in a Phase I clinical trial in HIV-uninfected adults.
机构信息
Infectious Diseases Division, University of Rochester Medical Center, Rochester, NY 14642, United States. xia
出版信息
Vaccine. 2009 Nov 23;27(50):7080-6. doi: 10.1016/j.vaccine.2009.09.060. Epub 2009 Sep 26.
Abstract
A Phase I human vaccine trial of a novel polypeptide vaccine of HIV T helper epitopes (EP-1043) and a DNA vaccine of HIV CTL epitopes was conducted in 84 healthy adult volunteers. The vaccine immunogenicity was assessed by an intracellular cytokine staining assay for IL-2, IL-4, TNF-alpha and IFN-gamma. Sixty eight percent (32/47) of subjects had a positive CD4+ T response after receiving two vaccinations of the polypeptide vaccine. The responding CD4+ T cells made various combinations of IL-2, IL-4, IFN-gamma, and TNF-alpha. The study demonstrated that the EP-1043 vaccine is safe, well-tolerated, and immunogenic.
摘要
一项新型 HIV 辅助表位（EP-1043）多肽疫苗和 HIV CTL 表位 DNA 疫苗的 I 期人体疫苗试验在 84 名健康成年志愿者中进行。通过细胞内细胞因子染色测定法评估疫苗的免疫原性，以检测 IL-2、IL-4、TNF-α和 IFN-γ。接受两次多肽疫苗接种后，68%（32/47）的受试者出现 CD4+T 反应阳性。应答的 CD4+T 细胞产生了各种组合的 IL-2、IL-4、IFN-γ和 TNF-α。该研究表明，EP-1043 疫苗安全、耐受良好且具有免疫原性。
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