生物膜与慢性鼻-鼻窦炎患者鼻窦组织中的TH1炎症相关。
Biofilms correlate with TH1 inflammation in the sinonasal tissue of patients with chronic rhinosinusitis.
作者信息
Hekiert Adrianna M, Kofonow Jennifer M, Doghramji Laurel, Kennedy David W, Chiu Alexander G, Palmer James N, Leid Jeffery G, Cohen Noam A
机构信息
Department of Otorhinolaryngology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
出版信息
Otolaryngol Head Neck Surg. 2009 Oct;141(4):448-53. doi: 10.1016/j.otohns.2009.06.090.
OBJECTIVE
Determine the prevalence of bacterial biofilms in surgical chronic rhinosinusitis (CRS) patients and characterize the inflammatory response associated with biofilm CRS.
STUDY DESIGN
Cross-sectional.
SETTING
Tertiary care academic center.
SUBJECTS AND METHODS
Sinonasal mucosa and peripheral blood were collected from 60 CRS patients. Mucosal biofilms were demonstrated by scanning electron microscopy. Leukocyte subpopulations were determined by flow cytometry. Cytokines were identified with a luminex-based assay on the lysate of homogenized tissue or plasma.
RESULTS
Of the 60 samples, 17 were determined to be positive for the presence of biofilms. Oral steroid-naive CRS patients with biofilm demonstrated a local T(H)1 inflammatory response with significantly elevated levels of interferon-gamma (INF-gamma), granulocyte colony-stimulating factor, macrophage inflammatory protein-1 beta, and neutrophils in the sinonasal mucosa. No differences were present at the systemic level.
CONCLUSION
Sinonasal bacterial biofilms correlate to a T(H)1 skewed local but not systemic inflammatory response in CRS. This difference is abrogated by the use of oral steroids.
目的
确定手术治疗的慢性鼻-鼻窦炎（CRS）患者中细菌生物膜的患病率，并描述与生物膜性CRS相关的炎症反应特征。
研究设计
横断面研究。
研究地点
三级医疗学术中心。
研究对象与方法
收集60例CRS患者的鼻窦黏膜和外周血。通过扫描电子显微镜观察黏膜生物膜。采用流式细胞术测定白细胞亚群。使用基于荧光微球技术的检测方法对匀浆组织或血浆裂解物中的细胞因子进行鉴定。
结果
在60份样本中，有17份被确定存在生物膜阳性。未使用口服类固醇的生物膜性CRS患者鼻窦黏膜呈现局部T(H)1炎症反应，干扰素-γ（INF-γ）、粒细胞集落刺激因子、巨噬细胞炎性蛋白-1β和中性粒细胞水平显著升高。全身水平未见差异。
结论
鼻窦细菌生物膜与CRS中局部T(H)1偏向性炎症反应相关，但与全身炎症反应无关。使用口服类固醇可消除这种差异。
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