Park Seong Kook, Cho Hong Wook, Heo Kyung Wook, Hur Dae Young, Lee Hyun-Kyung
Department of Otorhinolaryngology-Head and Neck Surgery, Inje University College of Medicine, Busan Paik Hospital, Busan, Korea.
Otolaryngol Head Neck Surg. 2009 Oct;141(4):462-6. doi: 10.1016/j.otohns.2009.06.013.
Chitin is a recognition element for tissue infiltration by innate cells implicated in allergy and helminth immunity, and this process can be negatively regulated by vertebrate chitinases. Acidic mammalian chitinase (AMCase) and chitotriosidase (ChT) have chitinolytic activity, but little is known about their roles in nasal polyps.
A prospective controlled study.
A tertiary referral center.
Nineteen subjects with nasal polyps and 12 subjects with deviated nasal septums were recruited to obtain inferior turbinate mucosa samples. The expression levels of AMCase and ChT were compared in nasal polyp and inferior turbinate tissue samples. The tissue samples were analyzed by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining.
AMCase and ChT were detected in all nasal polyps and inferior turbinate tissues. AMCase and ChT messenger RNA and protein expression were significantly higher in nasal polyps than in inferior turbinate tissues. In nasal polyps, AMCase-positive and ChT-positive cells were detected in the epithelium, inflammatory cells, and submucosal gland.
AMCase and ChT may be important mediators in the pathogenesis of nasal polyps. Nasal polyps appear to have elevated levels of chitinases, and the presence or growth of chitin-containing pathogens might enhance chitinase expression, resulting in nasal polyp formation and growth in susceptible individuals.
几丁质是参与过敏和蠕虫免疫的天然细胞进行组织浸润的识别元件，这一过程可受到脊椎动物几丁质酶的负调控。酸性哺乳动物几丁质酶（AMCase）和壳三糖苷酶（ChT）具有几丁质分解活性，但它们在鼻息肉中的作用鲜为人知。
一项前瞻性对照研究。
一家三级转诊中心。
招募了19名鼻息肉患者和12名鼻中隔偏曲患者以获取下鼻甲黏膜样本。比较鼻息肉和下鼻甲组织样本中AMCase和ChT的表达水平。通过逆转录-聚合酶链反应、蛋白质印迹法和免疫组织化学染色对组织样本进行分析。
在所有鼻息肉和下鼻甲组织中均检测到AMCase和ChT。鼻息肉中AMCase和ChT的信使核糖核酸及蛋白质表达显著高于下鼻甲组织。在鼻息肉中，在上皮细胞、炎性细胞和黏膜下腺中检测到AMCase阳性细胞和ChT阳性细胞。
AMCase和ChT可能是鼻息肉发病机制中的重要介质。鼻息肉似乎具有较高水平的几丁质酶，含几丁质病原体的存在或生长可能会增强几丁质酶的表达，从而导致易感个体中鼻息肉的形成和生长。
