Department of Otorhinolaryngology, China Medical University Affiliated Shengjing Hospital, Shenyang City, China.
J Otolaryngol Head Neck Surg. 2011 Feb;40(1):64-9.
Acidic mammalian chitinase (AMCase) has been found to play an important role in allergy and asthma. Chronic rhinosinusitis with nasal polyps (CRSwNPs) is chronic inflammation with eosinophilic infiltration, which is similar to asthmatic inflammation. The role of AMCase in CRSwNPs has been less studied. Eotaxin-3 is a potent eosinophil attractant and can induce eosinophil recruitment and activation in the airways of asthmatics. The expression and role of eotaxin-3 messenger ribonucleic acid (mRNA) and its relationship to AMCase in CRSwNPs have not been studied previously.
Twenty-three subjects with nasal polyps and nine subjects with nasal septum deviation were included in the study. The polyps and inferior turbinate mucosa were obtained as the tissue samples. Real-time reverse transcriptase-polymerase chain reaction was used to analyze and compare the expression levels of AMCase and eotaxin-3 in nasal polyps and inferior turbinate tissues (ITTs).
AMCase and eotaxin-3 were detected in all nasal polyps and ITTs. The expression ratio of AMCase mRNA was 123.90 ± 30.60 in nasal polyps and 2.38 ± 0.41 in ITTs. The expression ratio of eotaxin-3 mRNA was 43.58 ± 15.15 and 0.84 ± 0.30, respectively. The expression of AMCase and eotaxin-3 mRNA was significantly higher in nasal polyps than in ITTs (p < .01).
Our results reveal that AMCase and eotaxin-3 may be important mediators in the pathogenesis of nasal polyps. The increased AMCase and eotaxin-3 might lead to nasal polyp formation and growth. Future studies are needed to determine the potential of AMCase and eotaxin-3 as therapeutic targets in CRSwNPs.
酸性哺乳动物壳聚糖酶（AMCase）已被发现在过敏和哮喘中发挥重要作用。伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNPs）是一种慢性炎症伴嗜酸性粒细胞浸润，类似于哮喘炎症。AMCase 在 CRSwNPs 中的作用研究较少。嗜酸性粒细胞趋化因子-3 是一种有效的嗜酸性粒细胞趋化因子，可诱导哮喘患者气道中的嗜酸性粒细胞募集和激活。以前尚未研究过 CRSwNPs 中 eotaxin-3 信使 RNA（mRNA）的表达和作用及其与 AMCase 的关系。
本研究纳入了 23 例鼻息肉患者和 9 例鼻中隔偏曲患者。取息肉和下鼻甲黏膜作为组织标本。实时逆转录-聚合酶链反应用于分析和比较鼻息肉和下鼻甲组织中 AMCase 和 eotaxin-3 的表达水平。
在所有鼻息肉和下鼻甲组织中均检测到 AMCase 和 eotaxin-3。AMCase mRNA 的表达比值在鼻息肉中为 123.90±30.60，在下鼻甲组织中为 2.38±0.41。eotaxin-3 mRNA 的表达比值分别为 43.58±15.15 和 0.84±0.30。鼻息肉中 AMCase 和 eotaxin-3 mRNA 的表达均显著高于下鼻甲组织（p<0.01）。
我们的结果表明，AMCase 和 eotaxin-3 可能是鼻息肉发病机制中的重要介质。AMCase 和 eotaxin-3 的增加可能导致鼻息肉的形成和生长。需要进一步研究以确定 AMCase 和 eotaxin-3 作为 CRSwNPs 治疗靶点的潜力。
