哺乳动物双杂交技术日臻成熟。
Mammalian two-hybrids come of age.
机构信息
Department of Medical Protein Research, VIB, A. Baertsoenkaai 3, 9000 Ghent, Belgium
出版信息
Trends Biochem Sci. 2009 Nov;34(11):579-88. doi: 10.1016/j.tibs.2009.06.009. Epub 2009 Sep 26.
Abstract
A diverse series of mammalian two-hybrid technologies for the detection of protein-protein interactions have emerged in the past few years, complementing the established yeast two-hybrid approach. Given the mammalian background in which they operate, these assays open new avenues to study the dynamics of mammalian protein interaction networks, i.e. the temporal, spatial and functional modulation of protein-protein associations. In addition, novel assay formats are available that enable high-throughput mammalian two-hybrid applications, facilitating their use in large-scale interactome mapping projects. Finally, as they can be applied in drug discovery and development programs, these techniques also offer exciting new opportunities for biomedical research.
摘要
在过去的几年中，出现了一系列用于检测蛋白质-蛋白质相互作用的多样化哺乳动物双杂交技术，补充了已建立的酵母双杂交方法。鉴于它们在哺乳动物背景下运作，这些测定方法为研究哺乳动物蛋白质相互作用网络的动态提供了新的途径，即蛋白质-蛋白质相互作用的时间、空间和功能调节。此外，还提供了新型测定格式，可实现高通量哺乳动物双杂交应用，从而促进了它们在大规模相互作用组图谱绘制项目中的应用。最后，由于它们可应用于药物发现和开发计划，这些技术也为生物医学研究提供了令人兴奋的新机会。
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