来源于腐食酪螨的重组肌钙蛋白 C 的变应原性。
Allergenicity of recombinant troponin C from Tyrophagus putrescentiae.
机构信息
Department of Environmental Medical Biology and Institute of Tropical Medicine, Korea National Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Int Arch Allergy Immunol. 2010;151(3):207-13. doi: 10.1159/000242358. Epub 2009 Sep 29.
BACKGROUND
The storage mite, Tyrophagus putrescentiae, produces potent allergens, many of which have not been characterized. This study was undertaken to characterize the allergenicity of troponin C from T. putrescentiae.
METHODS
A cDNA encoding 17.7 kDa troponin C, with homology to cockroach allergen Bla g 6, was identified from T. putrescentiae-expressed sequence tags. Recombinant troponin C was expressed and IgE responses to the recombinant protein were assessed in the presence and absence of 10 mM CaCl(2). Cross-reactivity between T. putrescentiae troponin C and Bla g 6 was tested using an inhibition ELISA.
RESULTS
Recombinant T. putrescentiae troponin C shares 62.7-85.5% homology with troponin C from various arthropods. Sera from 5 of 47 subjects in our study group (10.6%) showed IgE binding to the recombinant protein. Interestingly, addition of 10 mM CaCl(2) increased the intensity of IgE binding approximately 2-fold. In an immune-inhibition ELISA with these sera, T. putrescetiae troponin C and Bla g 6 did not cross-react significantly.
CONCLUSIONS
Troponin C is a new mite allergen with calcium-dependent IgE reactivity.
背景
腐食酪螨会产生强过敏原，其中许多尚未被鉴定。本研究旨在鉴定腐食酪螨肌钙蛋白 C 的变应原性。
方法
从腐食酪螨表达序列标签中鉴定出与蟑螂过敏原 Bla g 6 具有同源性的 17.7 kDa 肌钙蛋白 C 的 cDNA。表达重组肌钙蛋白 C，并在存在和不存在 10 mM CaCl 2 的情况下评估其对 IgE 的反应。使用抑制 ELISA 测试腐食酪螨肌钙蛋白 C 与 Bla g 6 之间的交叉反应性。
结果
重组腐食酪螨肌钙蛋白 C 与来自各种节肢动物的肌钙蛋白 C 具有 62.7-85.5%的同源性。在我们的研究组中，47 名受试者中有 5 名（10.6%）的血清对重组蛋白具有 IgE 结合。有趣的是，添加 10 mM CaCl 2 使 IgE 结合的强度增加了约 2 倍。在使用这些血清的免疫抑制 ELISA 中，腐食酪螨肌钙蛋白 C 和 Bla g 6 之间没有明显的交叉反应。
结论
肌钙蛋白 C 是一种具有钙依赖性 IgE 反应性的新的螨变应原。
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