来自家食甜螨的一种新型2类变应原Gly d 2与来自粉尘螨、嗜卷书虱和腐食酪螨的2类变应原与重组变应原的交叉反应性研究

Cross-reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens.

作者信息

Gafvelin G, Johansson E, Lundin A, Smith A M, Chapman M D, Benjamin D C, Derewenda U, van Hage-Hamsten M

机构信息

Department of Medicine, Division of Clinical Immunology and Allergy, Karolinska Hospital and Institutet, Stockholm, Sweden.

出版信息

J Allergy Clin Immunol. 2001 Mar;107(3):511-8. doi: 10.1067/mai.2001.112264.

DOI:10.1067/mai.2001.112264

PMID:11240953

Abstract

BACKGROUND

Dust mites are important inducers of allergic disease. Group 2 allergens are recognized as major allergens in several mite species, including Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae. No allergens have thus far been characterized on the molecular level from the dust mite Glycyphagus domesticus.

OBJECTIVE

We sought to examine the cross-reactivity among group 2 allergens of G domesticus, L destructor, T putrescentiae, and D pteronyssinus.

METHODS

A group 2 allergen from G domesticus, Gly d 2, was cloned and expressed as a recombinant protein. Cross-reactivity between Gly d 2 and 3 other group 2 allergens, Lep d 2, Tyr p 2, and Der p 2, was studied by using individual sera and a serum pool RAST-positive to G domesticus, L destructor, T putrescentiae, and D pteronyssinus. Recombinant allergens were used as inhibitors of IgE binding in immunoblotting experiments. Molecular modeling on the basis of the Der p 2 structure was carried out for Gly d 2, Lep d 2, and Tyr p 2.

RESULTS

Two cDNAs encoding isoforms of Gly d 2 were isolated, but only the Gly d 2.02 isoform was used in this study. Sixteen of 17 subjects had IgE to Gly d 2. The protein sequence of Gly d 2 revealed 79% identity to Lep d 2 and 46% and 41% identity to Tyr p 2 and Der p 2, respectively. Extensive cross-reactivity was demonstrated among Gly d 2, Lep d 2, and Tyr p 2, but little cross-reactivity was found between these allergens and Der p 2. According to the tertiary structure of Der p 2 and 3-dimensional models of Gly d 2, Lep d 2, and Tyr p 2, differences reside mainly in surface-exposed residues.

CONCLUSION

Gly d 2 showed high sequence homology to Lep d 2. Cross-reactivity was observed between Gly d 2, Lep d 2, and Tyr p 2, but only limited cross-reactivity was demonstrated between these 3 allergens and Der p 2.

摘要

背景

尘螨是过敏性疾病的重要诱发因素。2类变应原被认为是几种螨类的主要变应原，包括屋尘螨、粉螨和腐食酪螨。迄今为止，尚未对家食甜螨的变应原进行分子水平的鉴定。

目的

我们试图研究家食甜螨、粉螨、腐食酪螨和屋尘螨2类变应原之间的交叉反应性。

方法

克隆家食甜螨的2类变应原Gly d 2，并将其表达为重组蛋白。使用个体血清和对家食甜螨、粉螨、腐食酪螨和屋尘螨呈RAST阳性的混合血清，研究Gly d 2与其他3种2类变应原Lep d 2、Tyr p 2和Der p 2之间的交叉反应性。重组变应原在免疫印迹实验中用作IgE结合的抑制剂。基于Der p 2结构对Gly d 2、Lep d 2和Tyr p 2进行分子建模。

结果

分离出两个编码Gly d 2异构体的cDNA，但本研究仅使用Gly d 2.02异构体。17名受试者中有16名对Gly d 2有IgE。Gly d 2的蛋白质序列与Lep d 2的同一性为79%，与Tyr p 2和Der p 2的同一性分别为46%和41%。Gly d 2、Lep d 2和Tyr p 2之间表现出广泛的交叉反应性，但这些变应原与Der p 2之间几乎没有交叉反应性。根据Der p 2的三级结构以及Gly d 2、Lep d 2和Tyr p 2的三维模型，差异主要存在于表面暴露的残基中。