一种高选择性的 ESIPT 荧光探针，用于双重特异性磷酸酶 MKP-6。
A highly selective fluorescent ESIPT probe for the dual specificity phosphatase MKP-6.
机构信息
Department of Chemistry, Dankook University, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do, 448-701, Korea.
出版信息
Chem Commun (Camb). 2009 Oct 21(39):5895-7. doi: 10.1039/b911145j. Epub 2009 Aug 17.
DOI:10.1039/b911145j
PMID:19787133
Abstract
A highly selective fluorescent probe for a protein tyrosine phosphatase (PTP) was designed by a simple phosphorylation of the 2-(2'-hydroxyphenyl)benzothiazole (HBT) chromophore: upon selective enzymatic hydrolysis, an excited-state intramolecular proton transfer (ESIPT) occurs, resulting in a large Stokes shift.
摘要
通过简单地对 2-(2'-羟基苯基)苯并噻唑（HBT）生色团进行磷酸化，设计了一种对蛋白质酪氨酸磷酸酶（PTP）具有高选择性的荧光探针：通过选择性的酶水解，发生激发态分子内质子转移（ESIPT），导致较大的斯托克斯位移。
Zotero 插件